地西泮在不同脑温下对癫行为和脑内氨基酸含量的影响

Effects of diazepam on seizure behavior and brain amino acid under different brain temperature

目的初步探讨地西泮抗癫作用温度效应及其基本机制。方法通过性发作行为记录的方法,观察地西泮在轻度高温、常温、亚低温状态下对大鼠性发作和脑组织内谷氨酸(Glu)、天门冬氨酸(Asp)和GABA含量的影响。结果与常温作用相比,在轻度高温状态下和亚低温状态下地西泮分别显著延长和缩短了性发作的持续时间,分别显著增强和减轻了性发作的强度;与常温作用相比,在轻度高温状态下和亚低温状态下地西泮分别显著增高和降低皮层及海马内Glu、Asp含量,并且,仅在亚低温状态下,地西泮显著增高皮层及海马内GABA的含量。结论地西泮在脑较高温度状态下不利于其抗癫作用的发挥,而在脑亚低温状态下显著提高其抗癫作用,其机制与脑温对脑内氨基酸含量的改变有关。

Objective To explore the effect of brain temperature on the anti-epilepsy effect of diazepam and its basic mechanism. Methods Seizure behavior was recorded and brain glutamate, aspartate and GABA were measured through HPLC technique in PTZ induced epileptic rats under under hyperthermia, normal temperature and hypothermia. Results As comparted with that under normal temperature, diazepam significantly prolonged and shorten the seizure duration under hyperthermia and hypothermia respectively; And as comparted with that under normal temperature, diazepam significantly increased and decreased the content of glutamate, and aspartate in cortex and hippocampus under hyperthermia and hypothermia respectively. But only under hypothermia, diazepam significantly increased the content of GABA in cortex and hippocampus. Conclusion hyperthermia reduces the anti-epilepsy effect of diazepam, while hypotherrnia enhances the anti-epilepsy effect of diazepam. The mechanism might be related to the alterations of brain amino acids under different temperature.