肺癌组织中内皮素1阳性表达及定量分析

Study on endothelin 1 positive expression and quantitative analysis in lung cancer

目的了解内皮素１（ＥＴ－１）在肺癌组织中表达及定量分析与肺癌分型、分级的关系。方法采用免疫组织化学ＡＢＣ染色及图像分析技术测定ＥＴ－１阳性率及含量。结果１０４例不同类型肺癌ＥＴ－１阳性表达主要分布在胞浆内，腺癌、鳞癌和大细胞肺癌ＥＴ－１表达较高，分别为７１．４％（２０／２８），５７．１％（１６／２８）和４０．０％（８／２０）。小细胞肺癌表达低为２１．４％（６／２８）。对腺癌、鳞癌阳性表达的图像分析表明，肿瘤分化程度越低ＥＴ－１含量越高，分化程度越高ＥＴ－１含量越低。结论ＥＴ－１普遍存在于肺癌细胞中，腺癌和鳞癌高表达，表明ＥＴ－１含量与肿瘤分化程度有一定关系，ＥＴ－１可作为腺癌、鳞癌恶性生长的一个监测指标。

Objective In order to study endothelin 1(ET 1) positive expression in lung cancer and the relationship between the ET 1 quantitative analysis and the types, grades of lung cancer.Method ET 1 positive expression and quantitative analysis were detected using avidin biotin peroxidase complex method and image analysis technology. Result The ET 1 positive expression were mainly located in the cytoplasm in 104 cases with various types of lung cancer. The positive rate of ET 1 in adenocarcinoma, squamous cell carcinoma and large cell lung cancer were 71.4%, 57.1% and 40.0% respectively. The positive rate of ET 1 in small cell lung cancer was 21.4%, significantly lower than others. The results of image analysis on adenocarcinoma and squamous cell carcinoma showed that the lower lung cancer differentiation, the higher ET 1 quantitative. Conclusion There is ET 1 positive expression in all of types of lung cancer, but there is a high ET 1 positive expression in adenocarcinoma and squamous cell carcinoma. The results of image analysis indicated that ET 1 quantitive expression was somehow related to the differentiation degree of neoplasm, so ET 1 quantitative analysis may be as a monitoring index of adenocarcinoma and squamous cell carcinoma growing.