生物材料影响巨噬细胞表达细胞因子的实验研究

An Experimental Study on the Cytokine Expression of Macrophage influenced by Biomaterials

探讨氧化钛(Titaniumoxide,Ti-O)薄膜和不锈钢(Stainless steel,SS)对巨噬细胞表达细胞因子的影响,以评价其生物相容性。将巨噬细胞分别与上述材料进行培养,并于72 h后提取一定量的上清液进行研究:(1)用NO试剂盒检测材料刺激巨噬细胞释放的一氧化氮(Nitride oxide,NO)的含量。(2)用酶联免疫吸附法(Enzyme linked immunosorbent assay,ELISA)检测单核细胞趋化蛋白-1(Monocyte chemoattractant protein 1,MCP-1)的含量。(3)用荧光显微镜计数细胞的数量,用扫描电镜(Scanning electron microscope,SEM)观察材料表面细胞的数量和形态。巨噬细胞在两种材料表面均有不同程度的生长与变形,细胞因子MCP-1的表达两材料间差异较大,Ti-O材料表面巨噬细胞数量最少,激活程度小,其表达的NO和MCP-1的含量也较低。材料表面巨噬细胞MCP-1以及NO的表达和变化与材料的生物相容性密切相关,经过本实验研究发现,Ti-O材料的生物相容性优于SS。

This experiment was designed to investigate the influence of two biomaterials, titanium oxide （Ti-O） and stainless steel （SS）, on the cytokine expression of macrophage, and further, to evaluate their biocompatibility. After being co-cultured with Ti-O and SS for 72 h, the cell number and morphology of macrophages attached on ma- terials were detected by fluorescent microscope and SEM. Nitride oxide （NO） and monocyte chemoattractant protein 1 （MCP-1） released by the macrophages co-cultured with different materials were also examined and compared. We found that the cell number of macrophages attached to Ti-O was smaller than that attached to SS. The levels of NO and MCP-1 released by the macrophages co-cultured with Ti-O were lower when compared with those released by macrophages co-cultured with SS. These results demonstrate that Ti-O has hetter bioeompatibility than does SS.