胶原蛋白耐湿热稳定性研究

Study on Hydrothermal Stability of The Collagen

天然胶原蛋白的热稳定性直接与其应用有关。利用两种收敛性比较弱的物质对胶原进行作用,使得产物的耐热稳定性得到大幅度提高,原因是这种作用产生了协同效应。实验中对合成的水杨酸-金属配合物采用紫外、红外、核磁共振等谱学方法进行结构分析,用配合物对皮粉进行作用,利用差示量热扫描法(Differential Scanning Calorimeter,DSC)测定鞣后皮粉的相变温度,推测配合物在皮粉内以刚性模块的形式对提高成胶原耐热稳定性起着重要的作用。结果发现:稳定常数较大的水杨酸铬在提高皮胶原纤维的热稳定性能力最强,其作用后皮粉的热变性温度为146.7℃,其次是水杨酸铝,为145.7℃。

The low hydrothermal stability of the raw collagen restricts its usage. To improve the hydrothermal stability of collagen, two kinds of materials with weak astringency were used by experts. The research proved that the synergistic effect was formed during the process. In this study, by using UV, FT-IR, laCNMR spectra and ele- mental analysis on the salicylic acid and metal-salicylic complexes, we could get the structural formula of every com- pound. And then, the hide powder was treated with the compounds. At last, the treated hide powder was tested by DSC. It could be presumed that the Rigid Matrix formed between the collagen doses can increase the hydrothermal stability of raw collagen, The result indicated that salicylic-chrome with large stable constant was better than others in improving the heat resistance of raw collagen, and the denaturalization temperature of hide powder treated with salicylic-chrome was 146.7℃. Salicylic-aluminum was in the second place, the relevant temperature being 145.7℃.