端粒长度与腰骶部慢性骨筋膜间隔综合征

Telomere length and chronic compartment syndrome in the lumbosacral region

背景:端粒的长度与细胞老化、永生化、癌变有密切的关系。目的:探讨端粒长度变化在腰骶部慢性骨筋膜间隔综合征中的意义。设计、时间及地点:对比观察,实验于2007-07/2008-02在上海交通大学医学院医学基因组学国家重点实验室完成。对象:25份竖脊肌组织标本取自2007-05/09,年龄29～49岁,平均(34.7±12.6)岁,其中10份为正常人标本,15份为腰骶部慢性骨筋膜间隔综合征所致慢性腰痛患者标本,根据年龄正常标本分为30～37岁和38～45岁2个对照组;非正常标本分为29～34岁、35～43岁和44～49岁组,每组5例。方法:取各组标本提取DNA,采用SouthenBlotting杂交技术,测量各组标本竖脊肌组织骨骼肌细胞端粒长度。主要观察指标:患者竖脊肌组织与正常组织端粒酶长度。结果:腰骶部慢性骨筋膜间隔综合征所致慢性腰痛患者竖脊肌细胞平均端粒长度明显比正常人竖脊肌细胞平均端粒长度短,且有显著性差异(P<0.05),44～49岁组最短,29～34岁组其次。结论:端粒的异常状态参与腰骶部慢性骨筋膜间隔综合征的发生。在腰骶部慢性骨筋膜间隔综合征病变的过程中,端粒长度明显变短,与年龄有关。

BACKGROUND： There is a close correlation between telomere length and cell senescence, immortalization or canceration. OBJECTIVE： To explore the significance of telomere length diversifies in the chronic compartment syndrome in lumbosacral region. DESIGN, TIME AND SETTING： A comparative observation was performed at the State-Level Key Medical Genomics Laboratory Shanghai Jiao Tong University School of Medicine from July 2007 to February 2008. PARTICIPANTS： The vertical spinal muscular tissue samples were taken from people aged 29-49 years old [mean aged （34.7±12.6） years] including 10 normal people and 15 patients with chronic compartment syndrome. According to the age, the normal were divided into the aged 30-37, and aged 38 45 groups. The abnormal were divided into aged 29 34, aged 35-43 and aged 44 49 groups, with 5 samples in each group. METHODS： DNA was extracted from samples of each group. Telomere length in vertical organizations spinal muscular skeletal muscle was measured by Southen Blotting hybrid technology. MAIN OUTCOME MEASURES： Telomerase length of erector spinal muscles in patients and normal people. RESULTS： The mean telomere length in patients with chronic compartment syndrome was obvious shorter than that of the normal people, which has significant difference （P〈 0.05）, the shortest was in the aged 44 49 and age 29-34 groups. CONCLUSION： The abnormal condition of telomere is involved in chronic compartment syndrome in lumbosacral region. Telornere length becomes shorter in the process of chronic compartment syndrome with related to the age.