OAS-1基因SNP rs10774671与慢性HBV感染者自发性HBeAg血清转换的关系

Relationship between SNP rs10774671 on OAS-1 gene and spontaneous HBeAg seroconversion in chronic HBV infection

目的探讨OAS-1基因SNP rs10774671与慢性HBV感染者自发性HBeAg血清转换的关系。方法收集慢性HBV感染者HBeAg阳性组58例、anti—HBe阳性组68例和无HBV感染对照组72例的外周血，进行DNA提取扩增，再用竞争分化聚合酶链反应方法扩增，结合酶免疫显色检测该SNP的基因型，组间差异进行分析比较。结果HBeAg阳性组的基因型GG＋GA比例为31．0％、等位基因G频率为16．4％，而anti-HBe阳性组为48．5％、29．4％，对照组为50．0％、28．4％；HBeAg阳性组与anti-HBe阳性组或对照组之问的差异均有统计学意义，而anti-HBe阳性组与对照组之间的差异无统计学意义。结论SNP rs10774671等位基因G可能有助于慢性HBV感染者发生自发性HBeAg血清转换，其基因型检测对于慢性HBV感染者干扰素治疗有一定的预测价值。

Objective To investigate the relationship between the SNP rs10774671 on OAS-1 gene and spontaneous HBeAg seroconversion in chronic HBV infection. Methods Blood samples were collected from 58 HBeAg positive, 68 anti-HBe positive patients with chronic HBV infection, and 72 normal control cases without HBV infection. Chromosomal DNA was extracted and OAS-1 gene was amplified. SNP genotyping was performed with the competitively differentiated polymerase chain reaction and enzyme immunoassay. Results In HBeAg positive group, frequencies of genotype GG plus GA and allele G were 31.0% and 16.4%. They were 48.5% and 29.4% in anti-HBe positive group, and 50.0% and 28.4% in normal control group respectively . Differences between HBeAg positive group and anti-HBe positive group or normal control group were statistically significant. But they weren＇t between anti-HBe positive group and normal control group. Conclusion Allele G on SNP rs10774671 of OAS-1 gene maybe benefits patients with chronic HBV infection to achieve spontaneous HBeAg seroconversion. Genotyping on this SNP may be predicting valuable for interferon therapy for chronic HBV infection.