摘要
目的通过体外实验,观察利巴韦林注射液对肠道病毒71型(EV71)病毒在横纹肌肉瘤传代细胞(RD.A细胞)复制的抑制作用和体外灭活作用。方法使用在2008年4月安徽阜阳疫情中分离到的EVT1病毒,通过三种体外细胞实验:药物在细胞中抑制EVT1病毒复制的药效测定实验;药物对细胞的保护作用实验;药物体外对EV71病毒的灭活作用的药效测定实验。观察利巴韦林注射液对EV71病毒感染横纹肌肉瘤传代细胞(RD—A细胞)中所起的作用。结果在利巴韦林注射液在细胞中抑制EVT1病毒复制的药效测定实验中,1:640利巴韦林注射液稀释度组较病毒对照组延迟病变出现2d,细胞生长未受到影响。在药物对细胞的保护作用实验中,1:8利巴韦林稀释度组较病毒对照组延迟病变出现4d。在利巴韦林注射液药物体外对EV71病毒的灭活作用的药效测定研究中,1:40稀释度组较病毒对照组延迟病变出现2d,而细胞生长未受到影响。结论利巴韦林注射液在体外有抑制EVT1病毒复制和部分灭活病毒的作用;并有一定的保护细胞抑制EV71病毒感染的作用。本研究对于临床上EV71感染的早期预防和治疗具有一定的指导意义。
Objective Observe the effect of Ribavirin on reducing the EV71 replication, inactivating EV71 and protecting the RD-A cells against the EV71 infection in vitro. Methods Using the EV71 isolated from Anhui Fuyang HFMD outbreak, the effect of Ribavirin on RD-A cells during the EV71 infection was observed. Results In the experiment of Ribavirin inhibiting the EV71 replication, comparing with the no-Ribavirin-dealed virus control group, the group of 1 : 640 dilution of Ribavirin delayed the CPE for 2 days, while the normal cell group was growing very well. In the experiment of protecting cell from EV71 infection, comparing with the no- Ribavirin-dealed virus control group, the group of 1:8 dilution of Ribavirin delayed the CPE for 4 days. In the experiment of Ribavirin effect on the inactivation of EV71, the group of 1:40 dilution of Ribavirin delayed the CPE for 2 days comparing with the virus control group. Conclusion Ribavirin could inhibit the replication of the EV71 and inactivate the EV71 in vitro. Additionally, Ribavirin could protect RD-A cells from EV 71 infection. The observation will contribute to EV71 infeetion control and quick medicine therapy.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2009年第1期44-46,共3页
Chinese Journal of Experimental and Clinical Virology