HBV感染患儿外周血NK及NKT细胞分析与临床意义

Peripheral NK and NKT cell in pediatric subjects with chronic HBV infection

目的探讨慢性乙型肝炎（CHB）患儿外周血NK及NKT细胞变化特点及与肝病病情的关系。方法采用流式细胞术对CHB患儿42例、正常儿童15例的外周血NK细胞及NKT细胞进行检测，并结合血清转氨酶（ALT）和病毒载量水平进行统计学分析。结果CHB患儿外周血NK细胞与正常儿童相应的细胞群比较，差异有统计学意义（P〈0．05），而NKT细胞则无统计学（P〈0．05）。结论CHB患儿外周血NK细胞频数较正常儿童明显升高。

Objective To investigate the characteristic of NK cells and NKT cells in HBV infected pediatric subjects for evaluation of their clinical implication. Methods Fresh peripheral blood samples were obtained from 42 HBV-infected pediatric cases and 15 healthy counterparts. NK cells and NKT cells were analyzed by flow cytometry assay. The clinical data such as serum ALT level and HBV viral load was simultaneously recorded from each HBV carrier. Results HBV-infected children had an obviously increasing percentage of NK cells 12.071% ± 7.100%, there were significant differences between the children with chronic B hepatitis and the healthy children （P 〈 0.05）. As far as percentage of NKT 3.048% ± 1.937% was concerned, there were not differences. Furthermore the association was not found between serum HBV viral load level and the NK lymphocyte. Conclusion Our data. may provide valuable information of NK and NKT lymphocyte for evaluation of disease progression of HBV infected children NKI cells.