鼻咽癌血清学检测方法的改进

Improve on serological diagnosis method of nasopharyngeal carcinoma

目的改进现有鼻咽癌血清学早期诊断方法，提高检测灵敏度。方法用链霉亲和素一生物素放大免疫酶法与既有常规免疫酶法对鼻咽癌防治示范基地所取294份普查血清IgA／VCA，IgMEA抗体进行血清学检测，并用SPSS统计软件对检测结果进行χ^2检验和t检验。结果共检测30岁以上294份普查人群血清，其中鼻咽癌患者血清106份，健康人血清188份。改进的链霉亲和素．生物素放大免疫酶法与既往已有的免疫酶法相比，血清IgMVCA、IgMEA抗体的检测阳性率增加；检测血清抗体的几何平均数明显提高。结论改进方法可以提高血清学检测的灵敏度，同时保证了检测结果的特异性，提高了鼻咽癌的检出率，可以应用于鼻咽癌早期筛查工作。

Objective To improve the existing serological early diagnosis method of nasopharyngeal carcinoma by improve the detection sensitivity. Methods The samples of 294 serum specimen from the prevention and treatment of nasopharyngeal cancer model base, involving 106 serum specimen from the patients suffering from nasopharyngeal cancer and 188 from tbe healthy testers. IgA/VCA antibody and IgA/EA antibody of the serums are tested by Streptavidin-biotin-antibody immunoenzymatic statistieal software is used to analyse the test results with test and normal traditional enzyme methods, SPSS χ^2 test and t test. Results Referring to 106 patients, the sera antibody positive rate tested by Streptavidin-biotin-antibody immunoenzymatic test method is obviously higher than that tested by traditional method ; and the t test result of the GMT has significant difference in the two method. Conclusion The modified method can improve the sensitivity of serology testing, ensure the specificity of test results, at the same time, improve the detection rate of nasopharyngeal carcinoma, so it can be applied to the early screen work of nasopharyngeal carcinoma.