摘要
丙型肝炎病毒HCV是非甲非乙型肝炎的主要病因，为单股正链RNA病毒，属黄病毒家族。全世界约有1．7亿人感染HCV，中国有大约4000万的HCV携带者^[1]。据预测，未来几十年中HCV感染人群还将继续增长。世界范围的高感染率，治疗手段和疫苗的缺乏使HCV成为研究的热点。一直以来，由于缺乏有效的HCV体外细胞培养体系，严重影响了HCV复制机制及新HCV防控策略的研究。2005年JFH1培养体系的建立突破了HCV不能体外培养的技术难题^[2]。在此基础上，HCV细胞培养技术取得突飞猛进的发展并广泛的应用于HCV相关研究中。
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2009年第1期76-78,共3页
Chinese Journal of Experimental and Clinical Virology
基金
国家863课题（2007AA022455）
自然科学基金（30571673,30671961）
参考文献17
-
1Alter HJ, Seeff LB. Recovery, persistence, and sequelae in hepatitis C virus infection: a perspective on long-term outcome. Semin Liver Dis,2000,20 : 17-35.
-
2Wakita T, Pietsehmann T, Kato T, et al. Produetion of infectious hepatitis C virus intissue euhure from a cloned viral genome. Nat Med, 2005,11 : 791-796.
-
3Lindenbach BD, Evans MJ, Syder A, et al. Complete replication of hepatitis C vlms in cell culture. Science, 2005,309:623-626.
-
4Pietschmann T, Kaul A, Koutsoudakis G, et al. Construction and characterization of infectious intragenotypic and intergenotypie hepatitis C virus chimeras. Proc Natl Acad Sci USA ,2006,103:7408- 7413.
-
5Gottwein JM, Scheel TK, Hoegh AM, et al. Robust hepatitis C genotype 3a cell culture releasing adapted intergenotypic 3a/2a ($52/ JFH1 ) viruses. Gastroenterology, 2007,133 : 1614-1626.
-
6Scheel TK, Gottwein JM, Jensen TB, et al. Development of JFI-II- based cell culture systems for hepatitis C virus genotype 4a and evidence for cross-genotype neutralization. Proc Natl Acad Sci U S A, 2008,105 : 997-1002.
-
7Yi M, Ma Y, Yates J, et al. Compensatory mutations in El, pT, NS2, and NS3 enhance yields of cell culture-infectious intergenotypic chimeric hepatitis C virus. J ViM, 2007,81 : 629-638.
-
8Mateu G, Donis R, Wakita T, et al. Intragenotypie JFH1 based recombinant hepatitis C virus produces high levels of infectious particles but causes increased cell death. Virol,2008,376:397-407.
-
9Delgrange D, Pillez A, Castelain S, et al. Robust production of infectious viral particles in Huh-7 cells by introducing mutations in hepatitis C virus structural proteins. J Gen Virol, 2007, 88 : 2495- 2503.
-
10Yi M, Pil|ez A, Castelain S, et al. Production of infectious genotype la hepatitis C virus (Hutchinson strain) in cultured human hepatoma cells. Proc Natl Acad Sci U S A,2006,103 : 2310-2315.
同被引文献35
-
1张连峰,李继昌,韩莉,成军,陈立东.丙型肝炎病毒非结构蛋白NS5A反式激活基因NS5ATP1的反式调节基因筛选[J].郑州大学学报（医学版）,2007,42(4):638-641. 被引量：2
-
2Yasukawa K, Konishi A, Inouye K. Effects of organic solvents on the reverse transcription reaction catalyzed by reverse transcriptases from avian myeloblastosis virus and Moloney murine leukemia virus [ J ]. Biosci Biotechnol Biochem, 2010, 74(9) : 1925 -1930.
-
3Cheng S, Fockler C, Barnes W M, et al. Effective amplification of long targets from cloned inserts and human genomic DNA [J], Proc Natl Acad Sci USA, 1994, 91 (12): 5695 -5699.
-
4Keeney S. Long-PCR amplification of human genomic DNA [ J]. Methods Mol Biol, 2011, 688 : 67 - 74.
-
5Sheehy P, Scallan M, Kenny-Walsh E, et al. A strategy for obtaining near full-length HCV cDNA clones (assemblicons) by assembly PCR [J]. J Virol Methods, 2005, 123(2) : 115 -124.
-
6Lu L, Nakano T, Smallwood G A, et al. A refined long RT-PCR tech- nique to amplify complete viral RNA genome sequences from clinical samples: application to a novel hepatitis C virus variant of genotype 6 [J]. J Virol Methods, 2005, 126(1/2) : 139 -148.
-
7Wang Y, Xia X, Li C, et al. A new HCV genotype 6 subtype designated 6v was confirmed with three complete genome sequences[ J ]. J Clin Virol, 2009, 44(3) : 195 -199.
-
8Timm J, Neukamm M, Kuntzen T, et al. Characterization of full- length hepatitis C virus genotype 4 sequences [ J]. J Viral Hepat, 2007, 14(5) : 330 - 337.
-
9Wakita T, Pietschmann T, Kato T, et al. Production of infectious hepatitis C virus in tissue culture from a cloned viral genome [ J ]. Nat Med, 2005, 11(7) : 791 -796.
-
10Fan X, Xu Y, Di-Bisceglie A M. Efficient amplification and cloning of near full-length hepatitis C virus genome from clinical samples[ J]. Biochem Biophys Res Commun, 2006, 346(4) : 1163 -1172.
引证文献2
-
1郭艳,兰林,何长龙,洪国祜,程玲,毛青.基于pJFH-1的HCV全基因组扩增方法的建立[J].第三军医大学学报,2011,33(11):1144-1147. 被引量：1
-
2卢莎,张玲,陶格斯,蔡敏,包丽丽,李恋,邓瑶,沈晓玲,谭文杰.嵌合中国河北株包膜蛋白基因的HCV细胞培养模型的传代分析[J].病毒学报,2015,31(6):647-652. 被引量：1
二级引证文献2
-
1何长龙,吴力克,兰林,洪国祜,张娟,张维,毛青.4种感染性腹泻病原菌免疫磁珠-多重PCR快速检测体系的建立[J].第三军医大学学报,2012,34(15):1505-1508. 被引量：3
-
2卢莎,谭文杰,张玲,邓瑶,陶格斯,蔡敏,李恋,沈晓玲.脂质体转染法与电转染法在丙型肝炎病毒RNA转染人肝癌细胞中的应用效果比较[J].山东医药,2016,56(19):15-18. 被引量：10
-
1乙肝病毒的突发型[J].吉林医学情报,1989(3):38-39.
-
2Grob,PJ,郝连杰.丙型肝炎病毒（HCV）,抗HCV,非甲非乙型肝炎[J].德国医学,1990,7(4):214-216.
-
3钟敏华,李文静,张勤奋,韩建德.衣原体持续感染诱导因素的研究进展[J].皮肤性病诊疗学杂志,2013,20(1):62-65.
-
4李新磊,周荣,彭涛.丙型肝炎病毒体外细胞培养系统研究进展[J].中国病毒病杂志,2015,5(1):58-66.
-
5王旭,程远国.丙型肝炎病毒细胞培养系统研究进展[J].生物技术通讯,2006,17(2):245-247.
-
6软骨细胞再生软骨新技术[J].中国科技信息,1999(9):78-78.
-
7梁紫岩,王宏卫.丙型肝炎DNA疫苗的研究进展[J].国外医学（预防．诊断．治疗用生物制品分册）,2002,25(6):242-245.
-
8欧武.丙型肝炎病毒的分子生物学研究进展[J].国外医学（微生物学分册）,1999,22(4):5-6.
-
9杨虹.妊娠合并戊型肝炎的有关进展[J].传染病信息,2007,20(5):283-286.
-
10李京涛,常占杰,席奇,刘亚珠,宋春荣,南然,李日向,魏海梁,闫曙光.HCV细胞培养系统及其在中草药提取物抗HCV研究中的应用[J].临床肝胆病杂志,2015,31(1):115-117. 被引量：4
