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反转录-聚合酶链式反应检测促血管生成素-1和促血管生成素-2及Tie-2受体的mRNA在血管瘤组织中的表达及其意义

Expression of Ang-1, Ang-2 and Tie-2 receptor mRNAs and their implications in hemangioma by RT-PCR
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摘要 目的研究血管瘤组织中促血管生成素-1（Ang-1）、促血管生成素-2（Ang-2）及Tie-2受体的mRNA表达水平，探讨其在血管瘤血管生成中的作用与意义。方法用反转录-聚合酶链式反应（RT—PCR）的方法分别检测30例血管瘤组织（增生期血管瘤17例，消退期血管瘤13例）以及10例小儿正常皮肤组织中Ang-1mRNA、Ang-2mRNA及Tie-2mRNA的表达水平。用免疫组织化学方法检测血管瘤组织Ki-67蛋白的表达。结果增生期血管瘤组织中Ang-2mRNA及Tie-2mRNA表达水平高于消退期血管瘤组织（P〈0．01）。血管瘤组织中Ang-1mRNA表达为阴性。结论Ang—Tie-2体系表达失衡在血管瘤的发生发展过程中可能起重要的作用。 Objective To detect the expression level of Ang-1, Ang -2 and Tie-2 receptor mRNAs in the hemangioma tissuses and to explore the effects of Ang-1, Ang-2, and Tie-2 receptor on the anglo- genesis in the hemangioma. Methods By use of serniquantinative reverse transcription polymerase chain reaction, we detected the expression level of Ang-1 mRNA, Ang-2 mRNA and Tie-2 mRNA in 30 cases of hemangioma （17 specimens of proliferating hemangioma, and 13 involuting hemangioma ）together with 10 cases of normal skin as control, and the expression level of Ki-67 protein was also detected by an immunohistochemiea method. Results The expression of Ang-2 mRNA and Tie-2 mRNA in the proliferating hemangiomas was higher than that in involuting hemangiomas （P〈0.01） . The expression of Ang-1 mRNA in hemangiomas was negative. Conclusion The unbalance of Ang-Tie-2 system is probably important for the pathogenesis and development in hemangiomas.
作者 洪帆 庄福连 王彪 林经安
机构地区 福建医科大学附属第一医院整形外科 福建医科大学附属第一医院中心实验室
出处 《中华医学美学美容杂志》 2009年第1期49-52,共4页 Chinese Journal of Medical Aesthetics and Cosmetology
基金 福建省教育厅科研基金资助项目（编号：JA05266）
关键词 血管瘤 促血管生成素 TIE-2受体 细胞增殖相关蛋白Ki-67 Hemangiornas Angiopoietin Tie-2 receptor Cell proliferation associated protein Ki-67
分类号 R732.2 [医药卫生—肿瘤]
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参考文献14

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二级参考文献39

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中华医学美学美容杂志
2009年 第1期

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