锁阳黄酮对大鼠运动耐力的影响及抗氧化作用

Effects of Cynomorium songaricum flavones on the exercise tolerance and antixidation in rats

目的探索锁阳黄酮(CSF)对大鼠运动耐力的影响及抗氧化作用。方法雄性Wistar大鼠分成5组(每组10只),分别为对照组,单纯游泳组,游泳(S)+CSF(2.0、1.0、0.5g/kg)3个剂量组,于动物每次游泳前1hig给药。采用循环水流自由游泳法,动物每天游泳1次,每次30min,连续10d,第11天进行运动耐力测试,用CuZn-SOD测试盒、MDA测试盒、紫外分光光度计检测血液中CuZn-SOD和MAD水平。结果与单纯游泳组相比,3个给药组于游泳前分别给予CSF0.5、1.0、2.0g/kg,动物体质量分别增长了8%、9.8%、12.5%;沉入水中前游泳时间分别增加了5.6%、16.7%、38.9%;每只动物10min平均下沉次数减少了19.2%、32.5%、55%;总游泳时间延长了5.9%、26.5%、51.5%;CuZn-SOD增加了1.1%、2.1%、2.7%;MDA降低了65.3%、83.9%、87.9%。结论CSF具有增强运动耐力、提高机体抗氧化、抗疲劳等作用。

Objective To study the effects of Cynomorium songaricum Rupr. flavones （CSF） on the exercise tolerance and the antixidation in rats. Methods Divide Wistar male rats into five groups （n= 10）, control group （1）, single swiming group （2）, swiming （S）＋CSF （2.0, 1.0, and 0. 5 g/kg） three dosage groups. The rats were administrated by ig before swiming each time. The rats swam daily in free Swimming in endless cycles water for 30 min, and for 10 d. The effects of the CSF on the swimming endurance of rats were investigated, the level of malondialdehyde （MDA）and CuZn-superoxide dismutase （CuZn-SOD）in blood were examined by MDA kit, CuZn-SOD kit, and ultraviolet spectrophotometer at 11 d. Results The results showed that the body weight of group 3,2, and 1 were increased significantly by 8 %, 9.8 %, and 12.5 % compared with to single swimming group （P〈0. 001）,respectively ;swimming time before sinking were increased by 5.6%,16.7%, and 38.9%, mean sinking times of each rat in 10 min were decreased by 19.2%, 32.5%, and 55% ; total swimming time were increased by 5.9% ,26.5%, and 51.5 % compared with to single swimming group （P〈0. 001）,respectively ;CuZn-SOD were increased by 1.1%,2. 1%, and 2.7%, MDA were decreased by 65.3%,83.9%, and 87.9%, in comparison with to single swimming group （P 〈 0. 001）, respectively. Conclusion CSF have effects of antixidation and increase the swimming endurance of rats.