木瓜蛋白酶提取草鱼皮胶原蛋白的工艺研究

Process Research of Extracting Collagen from Grass Carp Skin through Hydrolysis with Papain Proteinse

采用木瓜蛋白酶从淡水草鱼鱼皮中提取胶原蛋白,通过正交实验对草鱼皮胶原蛋白提取工艺进行优化。结果表明,料液比为1g/20ml、酶与原料比为1∶50、在pH值为6.0、酶解36h时得到胶原蛋白提取率为13.98%。

Papain was used to hydrolyze collagen of grass carp skin. The optimum condition for papain to hydrolyze collagen of grass carp skin was： pH 6.0, the ratio of solid to liquid= 1g ： 20ml, m （papain） ： m （grass carp skin）= 1 ： 50 and hydrolyzation time=36h.