普洱茶中没食子酸对过氧化物酶体增殖激活受体作用研究

STUDY OF GALLIC ACID IN PU-ERH TEA ON THE PEROXISOME PROLIFERATORS ACTIVATED RECEPTORS FUNCTION

目的研究从普洱茶中分离得到单体成分没食子酸对过氧化物酶体增殖激活受体(PPARα、PPARδ、PPARγ)作用的影响。方法首先确定没食子酸对三种细胞模型的加样浓度为50μg/ml,并以此浓度对激活PPARα、PPARδ、PPARγ受体的活性作用进行研究。结果没食子酸对PPARγ受体有激活作用,其激活倍数高达2.436,效果与阳性药物(2.438)相当;对PPARα、PPARδ受体的激活倍数0.816与1.303,基本上没有激活作用。结论普洱茶中没食子酸对PPARγ受体有激活作用,此结果可为普洱茶降糖降脂作用机制提供实验依据。

Objective To study the effect of gallic acid isolated from Pu-erh Tea on the peroxisome proliferators activated receptors function.Method The appropriate concentration of gallic acid added to three cell models was decided to be 50 μg/ ml,and the activity of gallic acid on peroxime prolipevators activated receptors PPARα,PPARδ,PPARγ was studied.Results Gallic acid could activate PPARγ,as high as 2.436 fold and the effect corresponded to that of positive drug which value was 2.438.gallic acid had no effect on PPARα and PPARδ.Conclusion Gallic acid in Pu-erh Tea had good activity on PPARγ and this could offer scientific basis for study of the anti-diabetes and anti-hyperlipidenmia mechanism of Pu-erh Tea.