一种快速分离小麦条锈菌转GUS基因转化体的方法

A Rapid Method to Separate the Transformants with GUS Gene in Puccinia striiformis

为了获得更多整合有外源GUS报告基因并且稳定表达的小麦条锈菌毒性突变菌株。探索小麦条锈菌夏孢子转GUS基因遗传转化体的分离方法。以从菌种筛选品种上分离转化体法为主、从繁殖品种辉县红上分离转化体法为辅可有效提高转化体的检出率;建立了利用透明胶带粘取夏孢子并通过染色分离小麦条锈菌转GUS基因转化体的新方法。试验证明,透明胶带粘取夏孢子染色法是一种快速有效分离小麦条锈菌转GUS基因转化体的方法。

To obtain more virulent mutant stains with GUS gene in Puccinia striiformis. The separation method of the transformants with GUS Gene in wheat stripe rust was studied. The result showed that to separate transformants from screening cultivars while at the same time separating transformants from breeding cultivar Huixianhong as an assistant way can increase the detectable rate efficiently. A new way to obtain the transformants with GUS gene through pasting and dyeing of urediospores of P. striiformis by transparent tape was established. The new method is an effective way to separate the transformants with GUS gene.