当归多糖铁小鼠口服的急性毒性研究

Acute toxicology of Angelica Sinensis polysaccharide-iron complex by oral administration in mice

目的:研究当归多糖铁(APIC)经口服途径给药的急性毒性。方法:将小鼠设5个剂量组,间距为0.64,进行LD50的预试,最大给药剂量为成人日常用药量的250倍,连续观察14d,对其体质、体质量、饮食等进行记录,在第14天解剖小鼠观察内脏有无明显损伤。结果:5组小鼠均未出现任何明显的急性毒性反应,无法测出半数致死量LD50,因此进行最大耐受量(MTD)测定,小鼠最大耐受量为4800mg·kg-1,小鼠最大耐受倍数为960。结论:动物急性毒理研究表明,APIC口服毒性甚小,临床应用安全可靠。

OBJECTIVE To observe the acute toxicology of Angelica Sinensis polysaccharide-iron complex（APIC） by oral administration in mice. METHODS Mice were divided into 5 groups and given with APIC at doses of 1 250, 800, 512, 409. 6 mg Fe/kg and water. The activity, body weight, water and food consumption were observed for 14 days. Histopathology was examined on the 14^th day. RESULTS No obvious acute toxicology appeared in all groups, so maximum tolerable dose （MTD） was used to be the index of toxicology instead of LD50. MTD of APIC in mice was 4 800 mg Fe/kg and it was 1 920 times of adult daily dose. CONCLUSION APIC for oral use is safe and of little toxicology.