阿司匹林对人γδT细胞和消化系统肿瘤细胞的作用比较

Comparison of aspirin on human γδT cells and digestive system's tumor cells

目的:比较阿司匹林对人γδT细胞和5株消化系统肿瘤细胞株的作用。方法:用不同浓度的阿司匹林诱导γδT细胞和消化系统肿瘤SGC-7901、SW-1990、SW-480、SW-1116、LOVO细胞株,用MTT法检测阿司匹林对这些细胞的抑制率和用乳酸脱氢酶法测定γδT细胞的杀伤活性,用流式细胞术检测诱导前后的γδT细胞和SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞凋亡率。结果:培养前γδT细胞数为5.12%,CD44表达5.13%;培养10d时γδT细胞数为91.27%,CD44为94.00%。阿司匹林在3.2mmol·L-1时对γδT细胞的生长抑制率达41.3%,明显高于对SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株抑制率(分别为19.6%,11.8%,9.2%,6.4%和1.6%)。0.4～1.6mmol·L-1阿司匹林诱导24h后的γδT细胞对5种肿瘤细胞的杀伤活性最高,浓度超过3.2mmol·L-1时杀伤活性呈下降趋势;SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞经不同浓度的阿司匹林诱导24h后γδT细胞对其杀伤活性除SW-1116和LOVO细胞株略有增强外,其他组与对照组比较无明显变化。3.2mmol·L-1阿司匹林对γδT细胞诱导24h的凋亡率(52.7%)显著高于SGC-7901、SW-1990、SW-480、SW-1116和LOVO细胞株(分别为7.9%,8.9%,6.2%,4.5%和3.7%)。结论:阿司匹林在临床常规使用的药物浓度时对5种肿瘤细胞株和γδT细胞无抑制作用,但能增强γδT细胞杀伤5种肿瘤细胞株活性,超过这一浓度可明显抑制γδT细胞的增殖能力和杀伤活性及增加γδT细胞的凋亡率,而对5种肿瘤细胞株作用不明显。这一结果为临床阿司匹林预防消化道肿瘤的用量提供了一定的参考价值。

OBJECTIVE To compare of the action of aspirin on human γδT cells and 5 digestive system＇s tumor cells. METHODS Various concentrations of aspirin were used to induce γδT cells and digestive system tumor cells SGC-7901, SW- 1990,SW-480, SW-1116,LOVO cells lines, MTT assays were used to detect aspirin on the inhibitory ratio of these cell lines, LDH assays were used to measure the cytotoxic activity of γδT cells, flow cytometry analysis for the apoptosis percentage of before and after induced γδT cells and SGC-7901, SW-1990, SW-480, SW-1116, LOVO cell lines. RESULTS γδT cells were cultivated for ten days which proliferation ratio increase from 5.12% to 91.27%, CD44 from 5.13% to 94. 0%. When aspirin＇s concentrations was 3. 2 mmol · L^-1 that γδT cells growth inhibitory ratio for 41.3% and which obvious higher than SGC- 7901, SW-1990, SW-480, SW-1116, LOVO cell lines （respectively 19. 6%, 11.8%, 9. 2%, 6. 4%, 1.6%）. When γδT cells induced via aspirin concentrations ranged from 0. 4 mmol·L^-1 to 1.6 mmol · L^-1 which cytotoxic activity on the five kinds of tumor cell lines was the highest, if aspirin＇s concentration surpassing 3. 2 mmol·L^-1, cytotoxic activity of γδT cells presented decrease tendency. SGC-7901, SW-1990, SW-480, SW-1116, LOVO cells lines were induced by different concentrations of aspirin for 24 hours, just SW-1116 and LOVO were enhanced as far as the cytotoxic activity of γδT cells on these cell lines was concerned, other groups and control group had no notable change. The apoptosis percentage of γδT cells（52. 7%） were induced by aspirin which concentration was 3. 2 mmol·L^-1, which is strikingly higher than SGC-7901, SW-1990, SW-480, SW-1116, LOVO cells lines, （respectively 7. 9%, 8. 9%, 6. 2%, 4. 5% and 3. 7%）. CONCLUSION When aspirin＇ s concentration for clinical routine used can enhance the effect of γδT cells killing the tumor cells, if surpassing this concentration can obvious inhibite the proliferation capacity and cytotoxic activity of γδT cells and augment apoptosis ratio, however, it is not obvious for digestive tract tumor lines. This result may offer an experiment base for applying clinical aspirin to prevent digestive tract＇s tumor.