小牛血清去蛋白注射液对2型糖尿病合并脑梗死模型大鼠胰岛素抵抗的影响

Effects of Deproteinised Calf Blood Injection on Insulin Resistance in Model Rat with Type 2 Diabetes Mellitus Complicating Cerebral Infarction

目的:观察小牛血清去蛋白注射液(DCBI)对2型糖尿病合并脑梗死模型大鼠血糖、血脂代谢的影响及其对胰岛素抵抗(IR)的改善作用。方法:大鼠用高脂高糖饮食伴尾静脉注射链脲佐菌素复制2型糖尿病模型,成功后再建立大鼠大脑中动脉栓塞模型,分为模型组、假手术组、DCBI(低、高剂量,30、60mg·kg-1·d-1,腹腔注射)组和对照药罗格列酮组;并设立正常组。各组给予相应试药5周后,测定空腹血糖(FBG)、血清胰岛素(FI NS)、糖耐量(OGTT)水平,并计算胰岛素抵抗指数(IRI)、胰岛素敏感指数(ISI),测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、游离脂肪酸(NEFA)水平。结果:与模型组比较,DCBI治疗后可显著降低2型糖尿病合并脑梗死模型大鼠FBG水平及增强糖耐量(P<0.01),对FI NS水平的改变不明显(P>0.05);改善机体对胰岛素的抵抗性(P<0.01或P<0.05);并明显降低TC、TG、LDL-C、NEFA水平及升高HDL-C水平(P<0.01),改善高脂状态。结论:DCBI可降低2型糖尿病合并脑梗死模型大鼠血糖,调节血脂代谢紊乱,具有改善IR的作用。

OBJECTIVE： To investigate the effects of deproteinised calf blood injection （DCBI） on blood glucose and lipid metabolism and its ameliorating effect on insulin resistance （IR） in model rats with type 2 diabetes mellitus （T2DM） complicating cerebral infarction. METHODS： Rats were fed with high fat and high glucose diet and injected with streptozotocin （STZ） vial caudal vein to duplicate T2DM model, followed by duplication of middle cerebral artery occlusion （MCAO） rat model. Then the rats were assigned to model group, sham-operated group, DCBI low and high dosage group （30, 60 mg·kg^-1·d^-1 intraperitoneally）, and rosiglitazone control group; meanwhile, another normal group was established. After receiving the corresponding drugs for 5 weeks for each group, fasting blood glucose （FBG）, serum insulin （FINS） and sugar tolerance （OGTT） were determined, and levels of insulin resistance index （IRI）, insulin sensitivity index （ISI）, total cholesterol （TC）, triglyeride （TG）, high-density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, and free fatty acid （NEFA） levels were computed. RESULTS： As compared with model group, in DCBI -treated model rats with T2DM complicating cerebral infarction, FBG were significantly decreased, sugar tolerance increased significantly （P〈 0.01）, yet the effect on the level of FINS was not significant （P 〉 0.05）; IR was ameliorated （P 〈 0.01 or P 〈 0.05）, levels of TC, TG, LDL- C and NEFA were significantly down regulated, HDL- C level was up - regulated （P〈 0.01） and the high lipid condition was ameliorated. CONCLUSION： DCBI can decrease the blood glucose level, regulate the disorder of lipid metabolism and ameliorate IR in model rats with T2DM complicating cerebral infarction.