高效液相色谱法测定人血浆中尼麦角林体内代谢物10α-甲氧基-9,10-二氢麦角醇的浓度

Determination of 10αMethoxy9,10dihydrolysergol

采用高效液相色谱法测定人血浆中１０α－甲氧基－９，１０－二氢麦角醇（ＭＤＬ，尼麦角林体内代谢物）的浓度．ＭＤＬ和麦角新碱（内标）用氯仿从血浆中提取，并在ＳｐｈｅｒｓｏｒｂＣ１８柱上，以乙腈—０．１ｍｏｌ／Ｌ（ｐＨ５）醋酸铵（１４）为流动相进行分离，在２２５ｎｍ处检测．本法简便、灵敏、专属性好．

A method for the determination of 10αmethoxy9,10dihydrolysergol(MDL),a nicergoline metabolite,in human plasma has been developed using HPLC.MDL and ergometrine as internal standard were extracted from the plasma with chloroform and separated on a reversed-phase ODS column with a mobile phase of acetonitrile-01mol/L(pH5)ammonium acetate(1∶4).The eluate was detected at 225nm,the method is simple,sensitive and specific.