一种简易的检测人体血浆中沙丁胺醇浓度的HPLC法

A Simple and Rapid HPLC Determination of Salbutamol Concentrations in Human Plasma with Fluorescence Detection

建立了一种简易的反相高效液相色谱方法测定沙丁胺醇血药浓度．以含磷酸二（２－乙基己基）酯的氯仿液进行提取，０．１ｍｏｌ／Ｌ盐酸反提，内标为丁酚胺．固定相为ＳｐｈｅｒｉｓｏｒｂＣ１８；流动相为：０．０３ｍｏｌ／Ｌ磷酸二氢铵（用磷酸调至ｐＨ２．６）—甲醇—乙腈（９２．２∶３．４∶４．４），流速１．５ｍＬ／ｍｉｎ．荧光检测器的激发波长为２２５ｎｍ，荧光波长为３１０ｎｍ．在血浆浓度０．５～５０ｎｇ／ｍＬ范围内线性良好．回收率为７８．３％～８３．６％．日内相对标准偏差为４．５％～６．８％，日间相对标准偏差为７．２％～９．５％．应用本方法测定了８名志愿者口服沙丁胺醇片后的血药浓度．

A simple and rapid HPLC method is presented for the determination of salbutamol in human plasma.Plasma samples were extracted with chloroform containing di(2ethylhexyl)phosphate.Samples were back extracted into a hydrochloric acid solution.This method involved a C18 column as stationary phase and 003mol/L (NH4)H2PO4(pH26,adjusted by H3PO4)-methanol-acetonitrile(922:34:44)as mobile phase.A fluorescence detector was used for this assay.Bamethane was used as the internal standard.The carlibration curve was linear between 05 and 50 ng/mL with a regression coefficient of 0999. The recoveries of salbutamol samples were 783%～836% for plasma. Inter-day and intra-day RSD were 45%～68% and 72%～95% respectively. This method has been used in the bioavailability study of salbutamol tablets (Ventolin, Glaxo) given to eight healthy volunteers.