胶束动电毛细管色谱法测定三磷酸腺苷制剂含量

Determination of ATP Preparation by Micellar Electrokinetic Capillary Chromatography

应用胶束动电毛细管色谱法测定三磷酸腺苷（ＡＴＰ）制剂的含量，同时还可测出所含二磷酸腺苷（ＡＤＰ）及一磷酸腺苷（ＡＭＰ）．测定运行液采用ｐＨ＝９．５的０．０２ｍｏｌ／Ｌ磷酸盐缓冲液，其中含０．１ｍｏｌ／Ｌ胆酸钠，恒定电压１６ｋＶ（运行电流１４５～１５５μＡ）．各组分的迁移时间（ｔＭ）在１０～２０ｍｉｎ之内．选择对氨基苯磺酸钠作内标，各组分浓度在０．１～２．０ｇ／Ｌ范围内对内标峰面积之比呈良好线性关系．样品各组分的批内ＲＳＤ＜３％（ｎ＝９），回收率为９９．１０％～１０１．４％，ＲＳＤ＜３％（ｎ＝５）．与药品标准的测定方法相比较，ＡＴＰ测定值最大相差为３．３％，但前者不能测定其它成分．

The contents of ATP in preparation are determined by micellar electrokinetic capillary chromatographic(MECC)method,the degradation products of ATP、AMP and ADP,may also be determined simultaneously.Using 002mol/L,pH=95 phosphate buffer containing 01mol/L sodium cholate as running solution and a constant voltage of 16kV(current 145～155μA),the migration times of individual component are 10～20 minutes.With sodium sulfanilate as internal standard,good linearity between the ratio of peak area of each component to internal standard and concentration over the range of 01～20g/L are obtained.The RSD of assay in a batch is less than 3%(n=9)and the recoveries of components are 9910%～1014% with RSD less than 3%(n=5),compared with the standard method,the maximum difference of results for 6 samples is less than 33%.