醒脑静注射液对脑出血大鼠IL-1β蛋白及其mRNA表达的影响

Effects of Xingnaojing Injection on the Expressions of the Protein of IL-1β and the mRNA in Rats with Intracerebral Hemorrhage

目的:研究醒脑静注射液对实验性脑出血大鼠IL-1β蛋白及其mRNA表达的影响。方法:参照邹氏、Rosenberg方法建立大鼠脑出血模型,分为正常、模型、醒脑静组。给药后观察各组细胞病理变化,检测IL-1β蛋白及其mRNA表达变化。结果:与模型组比较,醒脑静组细胞排列规整,水肿程度减轻,炎性细胞减少;线粒体结构破坏较轻,突触结构较清晰;IL-1β蛋白及其mRNA表达均极显著低于模型组。结论:醒脑静注射液能减轻脑出血大鼠血肿周围脑组织水肿,抑制血肿周围脑组织IL-1的表达。

OBJECTIVE： To study the effects of Xingnaojing injection on the expressions of both the protein of IL- 1β and the mRNA in the experiment intracerebral hemorrhage rats.METHODS： Referenced by Zou＇s method and Rosengerg method, rat intracerebral hemorrage model was established and the model rats were then divided into normal group, model group and Xingnaojing group .The pathological changes in all the groups were observed and the expressions of the protein of IL- 1β and the mRNA were detected after medication.RESULTS： In Xingnaojing group as compared with model group, the cells arranged regularly, the edema degree was lessened, the inflammatory cells were less; the disorganization of plastiosome was less severe, synaptic structure was clearer.The expressions of both the protein of IL - 1β and the mRNA were all lower than in the model group.CONCLUSION： Xingnaojing injection can relieve the cerebral edema and inhibit the expression of IL- 1β of the brain tissue surrounding the hematoma in intracerebral hemorrhage rats.