青蒿琥酯对人前列腺癌PC-3细胞内游离Ca^(2+)的影响

Effects of Artesunate on Free Ca^(2+) Concentration in Human Prostatic Cancer PC-3 Cells

目的:研究青蒿琥酯(ART)对人前列腺癌PC-3细胞内游离Ca2+浓度([Ca2+]i)的影响。方法:ART处理PC-3细胞,用Ca2+荧光探针Fluo-3/AM标记PC-3细胞,激光共聚焦扫描显微镜检测PC-3细胞内[Ca2+]i变化。结果:ART诱导PC-3细胞内[Ca2+]i在0～0.5h显著升高;在0.5～1h维持高水平;在4～24h降到初始水平。结论:ART能够诱导PC-3细胞内[Ca2+]i显著持续升高,[Ca2+]i升高可能在ART诱导PC-3细胞凋亡中起重要作用。

OBJECTIVE： To investigate the effects of artesunate（ART） on free Ca^2＋ concentration（ [Ca^2＋] i） in human prostatic cancer PC - 3 cells .METHODS：PC - 3 ceils were treated with ART and labeled using Ca^2＋ fluorescent probe Fluo - 3/ AM, and the change of [ Ca^2＋ ]i was evaluated by confocal laser scanning microscopy. RESULTS： After PC - 3 cells were exposed to artesunate, the intracellular [Ca^2＋]i concentration increased significantly at 0-0.5 h,maintained at a high level at 0.5-1 h, but decreased to the level of the initial at 4-24 h. CONCLUSION： ART can induce the marked sustained increase of[Ca^2＋] i and [ Ca^2＋]i might play an important role in ART - induced apoptosis of human PC - 3 cells.