摘要
目的:建立黄藤素缓释片在Beagle犬体内测定方法。方法:采用反相高效液相色谱法,色谱柱为Diamonsil ODS C18柱(250mm×4.6mm,5μm),流动相为乙腈-水(1∶1,每1000mL含NaH2PO43.4g,SDS1.7g),用H3PO4调节pH3,流速为1.0mL.min-1,柱温为35℃,检测波长为342nm。结果:血浆浓度在0.005～0.5μg·mL-1(r=0.9999)范围内与峰面积积分值呈良好线性关系,平均回收率为(102.22±2.48)%。结论:该方法操作简便,准确灵敏,重现性好,可满足黄藤素缓释片在Beagle犬体内的药动学和生物利用度研究。
OBJECTIVE：To establish an assay method for Sustained- release palmatine tablet in beagle dog. METHODS： Sample separation was performed on Diamonsil ODS C18 column（250 mm × 4.6 mm, 5μm） with mobile phase consisted of acetonitrile - water （ 1 ： 1, containing NaH2PO4 3.4 g and SDS 1.7 g in every 1 000 mL water, pH was adjusted to 3 by H3PO4） with a flow rate of 1.0 mL · min^- 1.The column was kept at 35 ℃ and the detective wavelength was set at 342 nm.RESULTS： The linear serum concentration range was 0.005--0.5μg · mL^-1（ r = 0.999 9） and the recovery rate was（102.22 ± 2.48）%. CONCLUSION ： The method was proved to be simple, accurate, sensitive and reproducible, and it was up to the standards for the study of pharmacokinetics and bioavailability of Sustained- release palmatine tablet in beagle dog.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第6期420-421,共2页
China Pharmacy
