RP-HPLC法测定薄荷脑樟脑滴鼻液中樟脑的含量

Determination of Camphor in Bohenao Zhangnao Nose Drops by RP-HPLC

目的:建立以反相高效液相色谱法测定薄荷脑樟脑滴鼻液中樟脑含量的方法。方法:色谱柱为Supelcosil C18(150mm×4.6mm,5μm),流动相为甲醇-水(70∶30),流速为1mL·min-1,柱温为25℃,检测波长为289nm。以外标法计算含量。结果:樟脑的检测浓度在0.1～1mg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9998);加样回收率为100%～103%,RSD<2%。结论:本方法简便、快速、准确,可用于薄荷脑樟脑滴鼻液的质量控制。

OBJECTIVE： To develop an RP HPLC method to determine the camphor in Bohenao zhangnao nose drops. METHODS： The HPLC separation was carried out on a Supelcosil C18（150 mm × 4.6 mm, 5μm） column.The mobile phase consisted of methanol -water（70 ： 30） at a flow rate of 1 mL · min^ -1.The column temperature was 25 ℃ and the detective wa- velength was set at 289 nm. The content was computed using external standard method. RESULTS： The linear range of camphor was 0.1-1 mg · mL^-1（r =0.999 8） with recovery rate ranged from 100% to 103% （RSD〈2%） . CONCLUSION： The method was proved to be simple, rapid and accurate,and it can be used for the quality control of Bohenao zhangnao nose drops.