反应条件对MTGase催化聚合WPC质构特性的影响

Study on texture characteristic of whey protein polymerized by MTGase with different condition

以乳清浓缩蛋白(WPC)为原料,探讨了微生物转谷氨酰胺酶(MTGase)催化聚合WPC对其质构特性的影响。结果表明:WPC经微生物转谷氨酰胺酶聚合后,在有还原剂二硫苏糖醇(DTT)存在的条件下,底物WPC质量分数达到8%,酶反应时间为2 h左右可以获得质构特性较好的凝胶。

Taken whey protein concentration as raw material of our experiment, we studied the effect of different condition to texture characteristic ofWPC polymerized by MTGase.The results showed that MTGase together with DTT in VCFC could form better gelatin when concentration of WPC reached 8% and the reactive time of MTGase reached 2 h.