摘要
建立了灵敏有效的测定婴幼儿配方乳粉中生物素质量分数的方法，用盐酸在超声波条件下除去蛋白质提取出生物素后，使用Hyper Sep C18固相萃取柱进行纯化、浓缩。采用荧光检测器，柱前PDAM衍生剂衍生，色谱柱为BDS HYPERSIL C18（250×4．6mm i．d．5μm），柱温为50℃，检测波长：Ex为340nm，Em为395nm。流动相流速1mL／min。标准曲线相关系数为0．9996；回收率为83．0％-101．5％之间；7次样品测定SD为0．45，RSD为2．35％。
A sensitive and reliable method was developed for the determination of biotin in infant formula milk powder. HCI was added into infant formula milk powder to move protein in ultrasonic wave condition and after solid-phase extraction （SPE） clean-up and concentration with HyperSep C18. Fluorscence detector, BDS HYPERSIL C18 （250×4.6 nun i.d. 5μm） column and derivation pre-column was used PDAM with column temperature 50℃, was measured fluorometricaly at the excitation and florescence wavelengths of 340 and 395 nm and speed of mobile phase 1.0 mL/min. The relative coefficient was 0.9996, the recovery 83.0%-101.5%; the SD in 7 samples by testing was 0.45 and RSD=2.35%.
出处
《中国乳品工业》
CAS
北大核心
2009年第2期53-56,共4页
China Dairy Industry
