降压胶囊中芍药苷的含量测定

Determination of Paeoniflorin in Jiangya Capsules by HPLC

目的:建立降压胶囊中芍药苷高效液相色谱测定方法。方法:色谱柱为Alltima C_(18)柱(250 mm×4.6mm,5μm),流动相为乙腈-水(13:87)。柱温:30℃,流速:1 ml·min^(-1),检测波长:230nm。结果:芍药苷在0.132～0.660μg范围内线性关系良好(r=0.999 9),回收率为101.3%,RSD=1.04%。结论:本方法简便、灵敏准确、重现性好,可用于控制降压胶囊的质量。

Objective： To establish an HPLC method for determination of paeoniflorin in Jiangya capsules. Method： The Alltima C18 column（250mm ×4.6mm,5μm） was used. The mobile phase was acetonitrile-water（13： 87）. The flow rate was 1ml·min^-1. The column temperature was 25℃. Detective wavelength was at 230nm. Result： The linear range of paeoniflorin was 0. 132 -0. 660μg, r = 0.999 9. The average recovery was 99.6%, RSD = 0.89% （ n = 5）. Conclusion ： The method was accurate, sensitive for the determination of Paeoniflorin in Jiangya Capsules.