淡水池养南美白对虾白斑综合症病毒的分离及其回攻试验

Physiology and attack-back research of white spot syndrome virus of litopenaeus vannamei boom reared in fresh-water

从苏州市某淡水养殖池塘中采得南美白对虾(Penaeus vannamei)病虾,从病体中分离出一种杆状病毒,纯化的病毒粒子经电镜观察发现其大小(50nm×375nm)、形态特征(杆状)与对虾白斑综合症病毒(WhiteSpot Syndrome Virus,简称WSSV)极其相似,克氏原螯虾(Procambarus clarki)回归试验及病毒分离、观察实验,初步表明采集的病料为对虾白斑综合症病虾.

A baculovirs was isolated from the virus-infected shrimp （penaeus vannamei） collected from a freshwater pond in Suzhou. The size （50nm × 375nm） and morphological characteristics （rod） of the purified virion was extremely similar to that of white spot syndrome virus （WSSV） by electron microscopic observation. The result of procambarus clarkii regression test and observation of isolated virus primaily indicated that the materials were shrimp white spot syndrome.