益筋方对实验性重症肌无力小鼠T细胞及IL-2的影响

Effect of Yi Jin Prescription on T Cells and IL-2 in Experimental Myasthenia Gravis Mice

目的探讨温肾活血、益气升清的益筋方对实验性自身免疫性重症肌无力(MG)小鼠的治疗机理。方法采用重症肌无力患者的血清被动免疫小鼠,建立实验性自身免疫性重症肌无力模型。分组后分别给予益筋方、强的松灌胃21d,处死动物、采血。利用流式细胞技术,以T细胞及其亚群、IL-2为指标,观察益筋方、强的松对实验性重症肌无力小鼠细胞免疫和细胞因子的影响。结果强的松、益筋方均能升高CD3+,降低CD4+/CD8+比值;降低IL-2。结论益筋方能通过影响T细胞及其亚群、细胞因子,调控细胞免疫和体液免疫,起到治疗重症肌无力的作用。

Objective To study the mechanism of Yi Jin Prescription treating the mice with myasthenia gravis（MG） caused by the disease of autoimmunity. Methods Experimental autoimmune myasthenis gravis mouse model was established with C57BL/6 mice immunized with plasma of myasthenia gravis. Mice were treated with prednisone and Yi Jin Prescription by intragastric administration and killed after 21 days. By using the flow cytometry, the effects of Yi Jin Prescription and prednisone on the T lymphocyte,T lymphocyte subgroups and IL-2 of the experimental myasthenia gravis mice were observed respectively. Results Both prednisone and Yi Jin Prescription increased the ratio of CD3^＋ T lymphocyte, and decreased the ratio of CD4^＋/CD8^＋ and the level of IL-2. Conclusion Yi Jin Prescription can regulate cytoimmunity and humoral immunity by influencing T lymphocyte and its subgroup and IL-2.