中药复方对模拟失重大鼠骨丢失的影响

Effects of Chinese Medicine Compound on Bone Loss of Weightlessness Rats Simulated by Suspension

目的观察中药复方对尾部悬吊模拟失重大鼠骨密度(BMD)、骨生物力学强度及组织形态计量学的影响。方法50只Wistar大鼠按随机区组实验设计法分成正常对照组、模型组及悬吊中药低剂量组、中剂量组、高剂量组,每组10只,实验周期21d。实验结束后,取右侧股骨和第4腰椎,用双能X线骨密度仪测量股骨、腰椎BMD;三点弯曲实验法及腰椎压缩实验法分别测定股骨和腰椎生物力学指标;取第3腰椎,制作不脱钙切片测量骨形态计量学指标。结果与正常对照组相比,模型组大鼠股骨、腰椎BMD明显降低(P<0.05),股骨最大载荷、弹性载荷、最大挠度、弹性挠度明显降低(P<0.05);腰椎最大载荷、弹性载荷明显降低(P<0.05);腰椎骨小梁体积百分比、骨小梁形成表面百分比、活性生成表面百分比、骨小梁矿化率明显降低(P<0.05)。与模型组相比,中药中剂量组可明显增加模拟失重大鼠股骨BMD和腰椎最大载荷、弹性载荷和腰椎骨小梁体积百分比、骨小梁形成表面百分比、活性生成表面百分比、骨小梁矿化率(P<0.05)。结论中药复方能促进骨的形成和矿化过程,增加BMD以及增强骨力学强度,从而达到防治骨丢失的作用。

Objective To study the effects of Chinese medicine compound on bone density, biomechanics, histomorphometry of weightlessness rats simulated by tail suspension. Methods Fifty Wistar rats were randomly divided into 5 groups with 10 rats each group： control group, model group, and low dose, medium dose and high dose Chinese medicine compound treated suspension group, the experiment period was 21 days. BMD of femur and lumbar vertebrae were detected by dual energy X-ray absorptiometry. The femoral biomechanics parameters and anti-compress ability of lumbar vertebrae were measured by three-point assay and compress test respectively. The quantitative structures of non-decalcified bone tissue sections were analyzed by histomorphometry. Result Compared with control group, BMD of femur and lumbar of model group decreased remarkably （P〈0.05）, Maximum Load, Elastic Load, Maximum Deflection and Elastic Deflection of femoral bone and Maximum Load and Elastic Load of lumbar vertebrae of model group decreased remarkably （P〈0.05）, TBV%, TFS%, AFS% and MAR% of lumbar vertebrae of model group also decreased remarkably （P〈0.05）. Compared with model group, BMD of femoral bone, Maximum Load and Elastic Load, TBV%, TFS%, AFS% and MAR% of lumbar of medium dose group increased remarkably （P 〈0.05）. Conclusion Chinese medicine compound can improve the bone formation, prevent bone loss by improving ossify, bone mineral deposition and mineralization, as well as increase BMD, improve the bone biomechanics property.