高效液相色谱法测定祖师麻中3种苯丙素成分的含量

Determination of Three Kinds of Phenylpropanoids in Stem Rind of Daphne giraldii by HPLC

目的建立以HPLC法同时测定祖师麻中瑞香素、7-OH香豆素、紫丁香苷含量的方法。方法采用YMC-PackODS色谱柱(250mm×4.6mm,5μm);以甲醇-0.5%醋酸-水(19︰8︰1)为流动相;双波长检测,λ1=327nm,λ2=266nm;流速为1.0mL/min;柱温30℃。结果3个组分均能达到基线分离,各组分的平均回收率在98.41%～100.18%之间。结论操作简便,结果可靠,重现性好,可作为该药材质量控制的方法。

Objective To establish a HPLC method for the determination of daphnetin, 7-OH coumarin and syringing in Zushima. Methods The separation was performed on YMC-Pack ODS （4.6 mm× 250 mm, 5 μm） column with a mobile phase consisting of methanol-0.5% acetic acid-water （19 ： 8 ： 1） as gradient elution at the flow rate of 1.0 mL/min. The dual detective wavelength was set at 327 nm and 266 nm. The column temperature was 30 ℃. Results Baseline separation was achieved for the analysis of the three index components. The average recoveries were 98.4%-100.2%. Conclusion This method is convenient, rapid and accurate. It can be used for quality control in production of Zushima.