摘要
目的建立复方茶碱麻黄碱片中可可碱的含量测定方法。方法采用高效液相色谱法,以KromasilC18(4.6mm×250mm,5μm)为色谱柱;流动相为0.05mol/L磷酸二氢钾溶液-甲醇-三乙胺(77∶23∶0.2);检测波长为215nm;柱温为45℃;流速为0.8mL/min。结果可可碱在0.3094~0.722μg(r=0.99996)浓度范围内呈良好的线性关系,平均回收率为100.49%,RSD=1.1%。结论本法可准确、灵敏、快速地测定复方茶碱麻黄碱片中可可碱的含量。
Objective To establish a HPLC method for determination of theobromine in Compound Theophylline and Ephedrine Tablet. Method HPLC method was adopted on Kromasil C18 column (250 mm×4.6 mm, 5 μm), and temperature was set at 45 ℃. The mobile phase consisted of 0.05 mol/L KH2PO4- Methanol- Triethanolamine (77 : 23 : 0.2), with flow rate of 0.8 mL/min. The UV detection wavelength was set at 215 nm. Results The calibration curves of theobromine was linear in the range of 0.3094- 0.722 ℃g (r=0.999 96), ant the average recovery was 100.49% (n=9) with RSD=1.1%. Conclusion This method is simple, rapid, sensitive and accurate, and can be used for determination of theobromine in Compound TheophyUine and Ephedrine Tablet.
出处
《中国中医药信息杂志》
CAS
CSCD
2009年第3期49-50,共2页
Chinese Journal of Information on Traditional Chinese Medicine
关键词
复方茶碱麻黄碱片
高效液相色谱法
可可碱
含量测定
Compound TheophyUine and Ephedrine Tablet
HPLC
theobromine
content determination