种植体早期边缘骨吸收的相关因素研究

The Relationship between TNFA-308 Gene Polymorphism and Marginal Bone Loss Around Dental Implants

目的探讨TNFA-308基因多态性、吸烟、年龄等因素是否与骨内种植体Ⅱ期手术前边缘骨吸收有关,探讨影响这种早期边缘骨吸收的原因,以期对临床有指导意义。方法采用病例对照研究,选取44例种植患者,根据有无早期边缘骨吸收分为阳性组和阴性组;同时收集所有患者的颊黏膜拭子提取DNA;采用聚合酶链式反应对TNFA-308进行基因型的测定。结果TNFA-308基因型分布两组间差异无统计学意义;而吸烟分布在两组间差异有统计学意义(P=0.027)。经多元logistic回归分析,得出吸烟患者发生骨吸收是不吸烟患者的8.403倍(P<0.05)。结论吸烟与早期边缘骨吸收发生有关,TNFA-308基因多态性不是边缘骨吸收发生的危险因素。

Objective The purpose is to evaluate the relationship between the genetic polymorphism of TNFA gene and the marginal bone loss around the implants before stage Ⅱ and to find the related factors influencing the marginal bone loss thus to predict the long stability of implants. Methods A ease-control study was used. Patients demonstrating marginal bone loss around implants at stage-Ⅱ surgery were designated as the inarginal bone loss （＋） ＇group and those without bone loss as the inarginal bone loss （-）＇group. Genomic DNA from buceal mueosa was amplified by the poly-merase chain reaction （PCR） to detect TNFA Gene. Results There were significant differences in the distributions of smoking between the two groups. However, the distribution of the TNFA-308 genotype was not significant in both groups. Multiple logistic regression shows the patients smoking had a 8.403 times greater likelihood of marginal bone loss than those without smoking. The differences between the other risk factors were non-significant. Conclusion Smoking is associated with early marginal bone loss and the TNFA-308 genotype in individual is not the risking factor of early marginal bone loss.