摘要
Ethanol extracts of brown seaweeds from Pakistan and China were isolated and compared for their antiallergenic activities. They included Sargassum tennerimum (ST) and Sargassum cervicorne (SC) from Pakistan, and Sargassum gramini- folium turn (SG), Sargassum thunbergii (STH), and Laminaria japonica (LJ) from China. The ethanol extracts of these brown seaweeds were optimized at 85% (v/v) ethanol for the maximum yield of phlorotannin, an inhibitor against hyaluronidase. Total phlorotannins contained in the crude extracts were measured as 1.71% (SG), 0.74% (STH), 0.97% (LJ), 3.30% (SC), and 5.06% (ST). The 50% inhibitory concentrations (IC50) of Pakistani SC and ST were 109.5 and 21 μg/ml, respectively, lower than those of Chinese SG, STH, and LJ (134, 269, and 148 μg/ml, respectively). An antiallergic drug, disodium cromoglycate (DSCG), had an IC50=39 μg/ml, and a natural inhibitor of hyaluronidase, catechin, had an IC50=20 μg/ml. The IC50 of ST extract was found similar to that of catechin (21 vs 20 μg/ml) and lower than that of DSCG (21 vs 39 μg/ml). This suggests that ST is a potent inhibitor of hyaluronidase, indicating a promising future development of natural antiallergic medicines or functional foods.
Ethanol extracts of brown seaweeds from Pakistan and China were isolated and compared for their antiallergenic activities. They included Sargassum tennerimum （ST） and Sargassum cervicorne （SC） from Pakistan, and Sargassum graminifolium turn （SG）, Sargassum thunbergii （STH）, and Laminariajaponica （LJ） from China. The ethanol extracts of these brown seaweeds were optimized at 85% （v/v） ethanol for the maximum yield ofphlorotannin, an inhibitor against hyaluronidase. Total phlorotannins contained in the crude extracts were measured as 1.71% （SG）, 0.74% （STH）, 0.97% （LJ）, 3.30% （SC）, and 5.06% （ST）. The 50% inhibitory concentrations （IC50） of Pakistani SC and ST were 109.5 and 21 μg/ml, respectively, lower than those of Chinese SG, STH, and LJ （134, 269, and 148 μg/ml, respectively）. An antiallergic drug, disodium cromoglycate （DSCG）, had an IC50=39 μg/ml, and a natural inhibitor of hyaluronidase, catechin, had an IC50=20 μg/ml. The IC50 of ST extract was found similar to that of catechin （21 vs 20 μg/ml） and lower than that of DSCG （21 vs 39 μg/ml）. This suggests that ST is a potent inhibitor of hyaluronidase, indicating a promising future development of natural antiallergic medicines or functional foods.
基金
Project supported by the Hi-Tech Research and Development Program(863)of China(No.2006AA09Z427)
the National Natural Science Foundation of China(Nos.30800859 and 30871948)
