二次回归正交设计优化紫茎泽兰中黄酮的提取工艺

Optimization of the Flavonoids Extraction from the Eupatorium adenophorum Spreng Using the Quadratic Orthogonal Regression Design

[目的]研究从紫茎泽兰中提取黄酮的最佳工艺条件。[方法]以黄酮的得率为目标,使用单因素试验方法考察乙醇浓度、提取温度、固液比以及提取时间对黄酮得率的影响,使用回归正交组合设计优化提取工艺。[结果]最优的黄酮工艺条件为:固液比为1∶30,提取时间为2.5 h,乙醇浓度为53%,提取温度为70℃。[结论]使用回归正交组合设计能合理地优化紫茎泽兰中黄酮的提取工艺,为紫茎泽兰的综合利用提供参考。

[ Objective] The aim of this study was to find the optimal extraction condition for the Eupatorium adenophorum spreng. [ Method] With the aim of extraction ratio of flavonoids, the single-factor experimental method was adopted to study on the influence such as mass concentration of ethanol,extraction temperature,solid-liquid ratio and extraction time,and the quadratic orthogonal regression rotational combinational design was used to optimize the extraction technique. [ Result ] The optimal condition is as follows： the solid to liquid ratio is 1：30, the extraction time is 2.5 hour, the tress contraction of ethanol is 53% and the extraction temperature is 70℃. [Conclusion] The experimental method can well optimize the technique and it also provides an appropriate way to synthetically utilize the Eupatarium adenophorum spreng.