培养条件对甘草不同外植体愈伤组织中黄酮形成的影响

Effects of Cultural Conditions on Flavonoids Formation in Different Explant Callus of Glycyrrhiza uralensis Fisch

[目的]筛选生产次生代谢产物黄酮的最佳外植体和培养基配方及培养条件。[方法]采用L1（4^5）正交试验设计和对比试验设计方法，研究ZT、ABA、Kr、6-BA和NAA5种外源激素在不同培养条件下对由甘草根和芽诱导的愈伤组织中黄酮形成的影响。[结果]测验表明，弱光照条件（100～300Ix）有利于黄酮产量的形成；根诱导的愈伤组织是黄酮产量形成的适宜外植体。F检验表明，5种外源激素对黄酮产量的形成存在极显著差异，对根诱导的愈伤组织中总黄酮含量的影响顺序是：KT〉6-BA〉ZT〉ABA〉NAA；对芽诱导的愈伤组织中总黄酮含量影响顺序是：6-BA〉KT〉NAA〉ABA〉ZT。经过优选获得最佳激素组合为：ABA 1．0mg／L＋KT 2．0mg／L＋ZT 1．0mg／L＋6-BA 1．0mg／L＋NAA 2．0mg／L.[结论]该研究为工业化生产次生代谢产物黄酮提供了理论依据。

[Objective] The research aimed to screen the best explant, medium formula and cultural conditions for producing flavonoids of secondary metabolites. [ Method ] Under different cultural conditions, the effect of 5 exogenous hormones of ZT, ABA, NAA, KT and 6-BA on formation of flavonoids in callns induced by roots and buds of Glycyrrhiza uralensis Fisch was studied by using the methods of orthogonal experiment design of L16（4^5 ） and contrast experiment design. [ Result] ]he T-test indicated that weak hght （100 - 300 Ix） was beneficial to forming of flavonoids production; the callus induced from mot was a suitable explant for foming of tlavonoids yield. F-test showed that 5 exogenous hormones had extremely significant difference on forming of tlavonoids production, the effect on total flavonoids content in the callus induced by root was in the such order as： KT 〉 6-BA 〉 zr 〉 ABA 〉 NAA; the effect on total flavonoids content in the callus induced by bud was in the such sequence as： 6-BA 〉 KT 〉 NAA 〉 ABA 〉 ZT. The optimal hormone combinations obtained after optimization were： ABA 1.0 mg/L ＋ KT 2.0 mg/L ＋ ZT 1.0 mg/L ＋ 6-BA1.0 mg/L ＋ NAA 2.0 mg/L. [Conclusion] The study provided a theoretical basis for industrialized production of secondary metabolites of flavonoids.