高效液相色谱法同时测定烟草中吡虫啉、多菌灵、甲基硫菌灵的残留量

Determination of Imidacloprid, Carbendazim and Thiophanate-methyl in Tobacco by High Performance Liquid Chromatography

用固相萃取-高效液相色谱法对烟草中吡虫啉(imidacloprid)、多菌灵(carbendazim)、甲基硫菌灵(thiophanate-methyl)3种农药同时进行残留分析。用丙酮/水(1:1,V/V)提取,二氯甲烷萃取,紫外检测器分析,检测波长为270 nm,流动相为甲醇/水(60:40,V/V)。试验结果表明,方法回收率吡虫啉为91.9%～97.1%,相对标准偏差为3.97%～10.01%;多菌灵为92.7%～99.9%,相对标准偏差为3.15%～5.37%;甲基硫菌灵为87.4%～95.3%,相对标准偏差为3.05%～4.61%。该方法操作简便,分离效果好,准确度和精密度均达到定量分析要求。

The residues of three pesticides （imidacloprid, carbendazim, thiophanate-methyl） in tobacco leaves were analyzed by using high performance liquid chromatography. The samples were distilled by acetone/water （v/v= 1：1）, extracted by dichloromethane and analyzed by using UV detector at 270nm. The liquid phase was methanol/water（60：40）. The analytical results showed that the recovery ratios of imidacloprid was 91.9%-97.1% and the relative standard deviations was 3.97%-10.01%, carbendazim was 92.7%-99.9% and the relative standard deviations was 3.15%-5.37%, thiophanate-methyl was 87.4%-95.3% and the relative standard deviations was 3.05%-4.61%.This method showed simple, good separation effect and high accuracy and precision met the demand of quantitative analysis.