摘要
目的:探讨益气活血化瘀法对大鼠肝癌前病变Caspase-3及组织学的影响。方法:160只雄性W istar大鼠随机分为空白组、模型组、中药高剂量组、中药低剂量组,用二乙基亚硝胺诱导肝癌的发生发展,以益气活血化瘀法对促肝癌大鼠进行治疗,采用比色法检测肝脏Caspase-3。结果:与模型组相比,中药高、低剂量组在12、16周时Caspase-3明显上调。结论:Caspase-3与肝癌的发生密切相关,益气活血化瘀法通过促进肝脏组织Caspase-3的表达,从而对减缓大鼠肝癌的形成有一定作用,同时,益气活血化瘀法对肝癌前病变肝组织亦有明显改善作用。
Objective: To investigate the effects of Yiqihuoxuehuayu method on the expression of Caspase-3 and histology in precancerous lesions of liver cancer in rats.Methods: One hundred and sixty male Wistar rats were randomly divided into blank control group,model control group,high dosage and low dosage group of Chinese Medicine.Hepatocellular carcinoma(HCC) was induced with the administration of Diethylnitrosoamine(DEN) in rats and treated with Yiqihuoxuehuayu method,Colorimetric method was used to measure Caspase-3 in liver.Result: as compared with model group,up-regulation of Caspase-3 occurred in high dosage group and low dosage group of Chinese Medicine at 12wk and 16wk.Conclusion: Caspase-3 is closely related to the occurrence of hepatocarcinogenesis,and Yiqihuoxuehuayu method can effectively alleviate the formation of hepatocarcinogenesis by accelerating the expression of Caspase-3 in liver tissue.Meanwhile,Yiqihuoxuehuayu method has obvious effect on the improvement of precancerous lesions of liver cancer.
出处
《中医药信息》
2009年第1期68-69,共2页
Information on Traditional Chinese Medicine
基金
黑龙江省自然科学基金资助项目(D2007-06)
