摘要
目的建立龙葵中澳洲茄胺的高效液相色谱（HPLC）检测方法。方法采用甲醇酸水超声提取龙葵生物碱，在回流条件下对生物碱苷进行酸性水解，得到相应的澳洲茄胺。然后进行高效液相色谱检测，色谱条件：色谱柱为Diamonsil C18色谱柱（250mm×4．6mm），乙腈-0．05％七氟丁酸（30：70）体系为流动相，流速为1．0ml／min，检测波长为210nm。结果龙葵生物碱获得了良好的基线分离；澳洲茄胺的线性范围为102～612μg·ml-1（r=0．9999）；加样回收率超过96．0％，RSD为3．35％（n=6）。结论该方法简单快速，准确可靠，可作为龙葵药材及其制剂的质量控制方法。该方法也为龙葵质量标准的制定及进一步的药效学研究提供了可靠的数据和方法学平台。
Objective To develop a new quantitative method for determining solasodine in Solanum nigrum L. by high - per- formanee liquid chromatography （HPLC）. Methods After sonic extracted with acidic methanol, the alkaloids were acid hydro- lyzed with reflux, and then eluted with acetonitrile -0.05% heptafluorobutyric acid as mobile phase. The chromatography param- eters were as follows： column： Diamonsil ODS analytical column; flow rate：1. 0 ml/min; detection wavelength： 210 nm. Results Baseline separation was obtained for the alkaloids. Good linear range was found in the concentration range of 102 - 612 μg/ml with r = 0. 999 9. The recoveries were no less than 96.0% , RSD was 3.35% （n = 6）. Coneltmion The proposed method is simple, fast, accurate and effective. It can be used as a powerful tool for quality control of Solanum nigrum L. , also prvides a scientific and technical platform for setting up a quality standard and for further pharmaceutical study.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第2期273-274,共2页
Lishizhen Medicine and Materia Medica Research
基金
江西省教育厅基金(No.赣教技字[2007]251)
