海南裂叶山龙眼叶超临界提取物化学成分的气相色谱-质谱联用分析(Ⅱ)

GC-MS Analysis of Extracts from the Leaves of Heliciopsis lobata by Supercritical Fluid(Ⅱ)

目的对海南裂叶山龙眼叶超临界20 MPa压力提取物进行分析,为进一步开发利用该资源提供依据。方法采用CO2超临界流体萃取海南裂叶山龙眼叶的脂溶性部位,气相色谱-质谱联用(GC-MS)法鉴定化学成分,归一化法测定各成分相对含量。结果从海南裂叶山龙眼叶超临界二氧化碳20 MPa压力提取物中共分离出11个组分,全部进行了鉴定,其中含量高于20%的有3个,为邻苯二甲酸二(2-乙基己基)酯(21.51%);二十九碳烷(25.73%);3,5-二烯豆甾烷(25.73%)。结论海南裂叶山龙眼叶的超临界二氧化碳20 MPa压力提取物中含有酯类、烃类、甾类等化学成分,为海南裂叶山龙眼的进一步研究奠定了物质基础。

Objective To analyze the constituents extracted from the leaves of Heliciopsis lobata by supercritical fluid CO2 （ SFE - CO2 ） at 20 MPa. Methods The constituents were extracted by supercritical fluid CO2 extraction method, then separated and identified by GC - MS. Relative content of each component was determined by area normalization. Results 11 separated constituents were identified, the contents of 3 compounds were over 20% . The main constituents in extracts were Bis （2 -ethylhexyl） phthalate （21.51%）, Nonacosane （25.73%）, Stigmastan-3,5- dien （25.73%）. Conclusion There are ester, hydrocarbon and steroid in the constituents extracted from the leaves of Heliciopsis lobata . The study established a good method for further study and development of Heliciopsis lobata.