木质素模型化合物与松柏醇-β-D-葡萄糖苷-[α-^13C]的聚合反应的研究

Study on Co-polymerization of Coniferin with Model Compound of Lignin

为了探讨造纸废水中残余木质素与木质素前驱物之间的聚合反应机理,研究了β-O-4型木质素模型化合物与木质素前驱物松柏醇-β-D-葡萄糖苷-[α-13C]的聚合反应方法及其反应机理。采用红外光谱、13CNMR波谱分析聚合产物的特性及结构,并用偏光显微镜对产物进行了观察;采用凝胶渗透色谱法(GPC)测定了产物的数均相对分子质量(Mn)。研究表明:在漆酶的催化作用下木质素前驱物松柏醇-β-D-葡萄糖苷与木质素模型物能很完全的发生聚合,产物中不存在起始物的晶体,Mn也大大的增加了。松柏醇-β-D-葡萄糖苷能与木质素模型物聚合,为进一步研究木质素前驱物松柏醇葡萄糖苷用于废水处理的研究奠定了基础。

In order to explore the mechanism of polymerization between lignin from effluent of pulping process and lignin precursors, the method and mechanism of co-polymerization of eoniferin with model compound of β-O-4 type lignin were investigated. The characteristics of polymerization product were analyzed by FT-IR, ^13C NMR and gel permeation chromatography （ GPC ）. Polarized microscopy was applied to observe the products. Moreover, GPC was used to determine mumber-average molecular mass （Mn ） of the polymerization product. The results showed that coniferin could completely polymerize with model compound of β-O- 4 type lignin to form lignin macromolecule. The Mn of the polymerized product was much higher than the starting materials. There was no crystalline starting material in the obtained polymer. These results could be the basis of waste-water treatment using coniferin and enzymes.