同步-导数固体基质室温磷光光谱法快速同时分析a-萘乙酸和6-苄氨基嘌呤

Rapid simultaneous analysis of α-naphthylacetic acid and 6-benzylaminopurine by synchronous-derivative solid-substrate room temperature phosphorimetry

研究了植物激素α-萘乙酸和6-苄氨基嘌呤混合物体系的同步-导数固体基质室温磷光光谱。在pH9.5的条件下,以1.5mol/L的KI-CH3 COOLi为重原子微扰剂,选择Δλ=180nm,在240～340nm的波长范围内对两者的混合物进行了同步磷光光谱扫描,并做一阶导数处理。结果表明,二者的同步磷光光谱得到了较好的分离,同步导数磷光峰分别位于257nm和305nm,可同时分别对其进行定量分析。α-萘乙酸和6-苄氨基嘌呤的检出限分别为14.88ng和6.75ng。方法可用于蔬菜中α-萘乙酸和6-苄氨基嘌呤的测定。

A method for the simultaneous determination of α-naphthylacetic acid and 6-benzylaminopurine by synchronous-derivative solid-substrate room temperature phosphorimetry was described. Various factors were studied including pH, the types and amounts of heavy atom salts and Δλ. With pH 9.5, 1.5 mol/L KI-CH3 COOLi as heavy atom salt, and Δλ = 180 nm, synchronous phosphorescence of the mixture was scanned in the wavelength range of 240- 340 nm, and transformed to first order derivative phosphorescence spectrum. By the synchronous-derivative method, the overlapped phosphorescence spectra were well separated, and the two synchronous derivative phosphorescence peaks located at 257 nm and 305 nm, could be used for the determination of these two auxins. For α-naphthylacetic acid and 6- benzylaminopurine, the linear ranges are 8 × 10^-6 - 9 × 10^-4 mol/L and 3 × 10^-6 - 1× 10^-3 mol/L, respectively, and the absolute detection limits are 14.88 ng/spot and 6.75 ng/spot respectively. The method is simple and expeditious, and it has been applied to the determination of α-NAA and 6-BAP in vegetable samples with satisfactory results.