乙型肝炎病毒cccDNA检测方法的建立及初步应用

Detection of hepatitis B viruse covalently closed circular DNA and its clinical application

目的:建立和应用聚合酶链反应法(PCR)检测慢性乙型肝炎患者肝组织中乙型肝炎病毒(HBV)共价闭合环状DNA(cccDNA)。方法:应用选择性PCR的方法检测30例乙肝患者肝组织中cccDNA,同时验证此方法的特异性。结果:31例慢性乙型肝炎患者肝组织中,10例HBVcccDNA阳性,阳性率为32.3%。HBV cccDNA阳性组的ALT、HBVDNA、和HBeAg水平均明显高于阴性组(P<0.01和P<0.05)。结论:该方法操作简单,特异性好,可用于测定肝组织中HBV cccDNA,用于了解乙肝患者病情和传染性强弱以及评价抗乙肝病毒药物的疗效。

Objective： To establish and apply PCR for detection of HBV cccDNA in hepatic tissue of patients with chronic hepatitis B. Methods： The hepatic tissue samples collected from 31 patients with chronic hepatitis B were detected for HBV cccDNA by selective PCR method, at the same time to verify this methods specificity. Results： Of the 31 patients, 10 were positive for HBV cccDNA detection and the positive rate was 32.3%. The levels of ALT, HBV DNA and HBeAg were significantly higher in the positive patients than those in the negative patients （P〈0.01, P〈0.05）. Conclusion： This method is proved to be specificity and easy to operate. The cccDNA detection is applied to understand the state of an illness and its infectivity in hepatitis B patients and to verify curative effect of medication.