正交试验优化新疆沙枣鞣质的提取工艺

Extraction Optimization Study of Tannin from Elaeagnus Angustifolia L. by Orthogonal Design

目的：建立沙枣中鞣质含量测定的方法，优选鞣质类成分的超声提取工艺。方法：采用磷钼钨酸-干酪素比色法，通过单因素分析法考察不同溶剂对沙枣总鞣质提取率的影响，采用正交试验设计，考察提取时间、提取次数和料液比3个因素时超声提取工艺的影响。结果：在760nm波长处，没食子酸的反应物在0．001～0.01mg．ml^-1浓度范围内与吸收度呈线性关系，r=0．9994。以70％丙酮为溶剂，超声提取3次，每次1．0h，料液比为1：10时，提取效果最好，干浸膏中总鞣质含量为6．32％。结论：该比色法可以作为沙枣鞣质的含量测定和质量控制的方法，优选工艺稳定，可行。

Objective：To establish a uv- vis method for determining the contents of tannin in elaeagnus angustifolia L. ,optimize the extracting process of tannins by ultrasonic wave.Methods： The Phosphomolybdium Tungsten Acid- Casein Reaction was used. The influences of different solvents to the extracting of tannin was tested by single factors, based on that the affects of 3 factors：dine for extracting, extracting times,material and solvent ratio to ultrasonic extraction technology were performed by orthogonal design.Results： The linearity of products was obtained within the range of 0.001- 0.01 mg· ml^- 1 （ r = 0. 9994）for Gallic acid at the wavelength of 760nm. The optimum extraction conditions were as follows：adding 10 - fold of 70% acetone,extracting 3 times,each time for 1 .Oh by uhrasonic wave.The average contents of total tannin in drying extract was 6.32%. Conclusion： The method established could be use for assay the contents of tannin in elaeagnus angustifolia L. and quality control, the technology optimized were steady and reproducible.