芪参活血颗粒联合氟康唑对深部白色念珠菌感染小鼠免疫功能的影响

Effect of Qishenhuoxue Granule Combined with Fluconazole on the Immune Function of Mice with Systemic C. albicans Infection

目的研究芪参活血颗粒联合氟康唑对深部白色念珠菌感染小鼠免疫功能的影响。方法建立小鼠深部白色念珠菌感染的动物模型,用随机数字表法分为空白对照组、模型组、中西药结合组和西药组,观察各组不同时间点实验动物血白介素-2(IL-2)水平及单核细胞表面MHCⅡ类分子表达水平的变化。结果芪参活血颗粒能显著增强单核细胞表面MHCⅡ类分子表达,提高外周血IL-2水平。结论芪参活血颗粒联合氟康唑治疗深部白色念珠菌感染的作用优于单纯使用氟康唑,芪参活血颗粒通过调节免疫力增强机体抗感染能力是其可能的作用机制之一。

Objective To detect the effect of Qishenhaoxue Granule（QHG） combined with fluconazole on the immune function of mice with systemic C. albicans（CA） infection. Methods Deep CA infection mice model were established by injecting standard strain of CA via caudal vein,and were divided into 4 groups at random, treated by gastrogavage with normal saline（ group B） , fluconazole（FCZ） ＋ QHG（ group C ）, fluconazole （FCZ groupD ） , and a blank group （group A ） with normal mice for control. The MHC Ⅱ positive rate expressed on monocyte and the content of interleukin 2 （IL-2） in blood serum were tested pretherapy and on the first day, the third day, the fifty day and the seventh day after treatment. Results QHG can increase MHC il expressed on the monocyte and the level of IL-2 in serum. Conclusion The effect of QHG combined with FCZ is better than that of FCZ only, and one of the possible leven mechanism is to strengthen anti-infection ability of mice by regulating immunologic system.