桃金娘油对人鼻腔黏液纤毛传输系统的影响

Effects of Myrtol Standardized on Human Nasal Mucociliary Transport

目的通过观察不同浓度桃金娘油对体外培养人鼻腔纤毛运动的作用,以及慢性单纯性鼻炎患者口服标准桃金娘油前后黏液纤毛传输时间的变化,探讨桃金娘油对人鼻腔黏液纤毛传输系统的影响。方法采用组织块法体外培养人鼻腔纤毛细胞,使用高速数字化显微成像技术,观察75μg/L、150μg/L和300μg/L的桃金娘油对纤毛摆动频率的影响。12名慢性鼻炎患者口服标准桃金娘油10d(300mg/次,3次/d),用药前后分别测定糖精清除时间,并对其中9名受试者用药前后分别进行相关鼻功能检查。结果①75μg/L、150μg/L和300μg/L的桃金娘油对体外培养的人鼻腔纤毛摆动频率未见显著影响。②12名慢性单纯性鼻炎患者口服药物后,糖精清除时间从用药前的(12.8±4.5)min缩短为(9.3±3.8)min(P<0.05)。③9名慢性鼻炎患者用药前后鼻阻力和鼻声反射结果差异均有统计学意义(P<0.05)。结论口服标准桃金娘油可改善鼻腔黏液纤毛传输系统功能和鼻腔通气功能,在体外未见75～300μg/L的桃金娘油对人鼻腔纤毛运动产生明显影响。

Objective To investigate the effects of Myrtol Standardized on the regulation of healthy human nasal ciliary beat frequency（CBF） and its influence on nasal mucociliary transport time（MTT）, Methods Changes in human nasal CBF in response to increasing concentrations of Myrtol Standardized within 20 minutes were quantified by using of high-speed digital microscopy. The MTTs before and after application of Myrtol Standardized in 12 patients with chronic rhinitis were determined by using the saccharin test. 9 patients were examined nasal function test. Results No statistically significant difference was identified when compared to basal CBF at the concentration of 75 ug/L, 150 ug/L or 300 ug/L, Myrtol Standardized. In addition, the use of Myrtol Standardized decreased the mean human nasal MTT from （ 12.8 ± 4.5 ） to （9.3 ±3.8 ） rain （ n = 12）. Conclusion Myrtol Standardized significaot/y increased MTT in vivo, but no obvious effect on human nasal CBF in vitro was observed.