槲寄生碱和多糖对肝癌细胞增生和凋亡的影响

Effects of Mistletoe Alkali and Mistletoe Polysaccharose Proliferation and Apoptosis of Hepato Carcinoma Cells

目的研究槲寄生碱和多糖对异型增生肝细胞DNA含量的影响。方法采用MTT比色法测定槲寄生碱(mistletoe alkali,MA)和多糖(mistletoe polysaccharose,MP)对SMMC-7721,HepG2细胞增生的影响。用流式细胞仪分析细胞周期、细胞凋亡,观察肝癌细胞的DNA相对含量,分析各时期细胞增生周期的移行变化。结果MA和MP有较好的抑制肝癌细胞增生的作用,呈现出时间-剂量依赖性;MP组及MA组SMMC-7721和HepG2细胞G1期比例增加(P<0.01),G2期和S期细胞比例降低(P<0.01),并使2种肿瘤细胞凋亡率增加(P<0.01),差异有统计学意义。结论MP和MA均能抑制人肝癌细胞SMMC-7721,Hep G2增生,促进其凋亡。

Objective To investigate the effects of Mistletoe Alkali（MA） and Mistletoe Polysaccharose（MP） on proliferation and apoptosis in hepatoma carcinoma cells. Methods In vitro experiments with mistletoe polysaccharides, and mistletoe alkali induced in hibition of SMMC-7721 and HepG2 proliferation were observed by MTF assay. The cell cycle, the rate of apoptosis and the DNA relative amount of SMMC-7721 and Hep G2 were examined by flow cytometry. The migrating changes of hepato carcinoma cell generation cycle were analyzed at every period. Results The results of in vitro experiments showed that both MA and MP could inhibit the proliferation of SMMC-7721 and Hep G2 cells, and promote their apoptosis in a time and dose-dependent manner. After treatment with the drugs, the cell population in the groups of MP and MA increased at G1 phase（81.0% , 86.9% vs 70.0% , P 〈0.01 ） and decreased at G2 phase and Sphase（13.1%, 5.7%vs 16.4%, P〈0.01; 5.9%, 7.4% vs 13.5%, P〈0.01）, in comparison with those in the controls. In addition, both of the two drugs enhanced the apoptosis of SMMC-7721 and Hep G2 cells （ MP： 12.3% , 11.7% vs 6.0 % , P 〈 0.01 ; MA ： 7.5% ,6.7% vs 6.0%, P 〈 0.01 ）. Conclusion MA and MP could inhibit the proliferation and promote the apoptosis of SMMC- 7721 and Hep G2 cells.