^125I-脱氧尿嘧啶核苷对淋巴瘤细胞Raji和Daudi的杀伤作用

Killing effect of ^125I-UdR on human lymphoma Raji and Daudi cell lines

目的探讨^125I-脱氧尿嘧啶核苷（^125I-UdR）在淋巴瘤细胞Raji和Daudi中的特异性摄取及其杀伤效应。方法测量Raft、Daudi细胞和细胞核在含不同放射性浓度^125I-UdR的培养液中培养不同时间后的活度；用噻唑蓝（MTT）实验和碘化丙啶（PI）染色周期分析评价^125I-UdR对Raji和Daudi细胞的杀伤作用。结果Raji和Daudi细胞摄取^125I-UdR的量明显高于Na^125I对照组（P〈0．05），在100kBq／ml浓度时，Raji和Daudi细胞摄取^125I-UdR的量分别为（14414±95）和（6916±53．69）Bq／10^6细胞，而对Na^125I的摄取量分别为（68±3．8）和（324±32．8）Bq／10^6细胞；细胞和细胞核中^125I-UdR的量随培养基中^125I-UdR放射性浓度以及培养时间的增加而增加；^125I-UdR组的存活分数明显低于Na^125I对照组（P〈0．05），以500kBq／ml浓度培养48h时，Raji和Daudi细胞^125I．UdR组的存活分数分别为（19．78±1．39）％和（43．17±2．69）％，而Na^125I组的存活分数分别为（79．10±1．79）％和（80．36±6．12）％；细胞存活分数有随培养基中放射性浓度增加而降低的趋势。结论^125I-UdR可被Raji和Daudi细胞特异性摄取并进入细胞核中，进而杀死细胞，其作用具有明显的时间-效应和剂量-效应关系。

Objective To evaluate the killing effect and the uptake of ^125I-UdR on human lymphoma Raji and Daudi cell lines. Methods The amount of ^125I-UdR in the cells and cell nuclei were determined after incubation of different time in RPMI 1640 culturing medium containing different concentrations of ^125I-UdR. The killing effects of ^125I-UdR on Raji and Daudi cell lines were estimated through MTF assay and cell cycle was analyzed by propidium iodide （PI） staining. Results The amounts of ^125I-UdR in Raji and Daudi ceils and cell nuclei were much higher than that of Na^125I（ P 〈 0.05）. The amounts of ^125I-UdR in Raji and Daudi cells were 14 414 ± 95 and （6916 ± 53.69） Bq/10^6 cell when the concentration was 100 kBq/ml. The amounts of Na^125I were 68 ± 3.8 and （324 ± 32.8） Bq/10^6 cell. The uptake of ^125I-UdR in Raji and Daudi cells and cell nuclei increased with the ^125I-UdR concentration and incubated time. The cell surviving fractions of ^125I-UdR groups was much lower than that of Na^125Igroups （P 〈 0.05）. When the concentration was 500 kBq/ml and incubated time was 48 hours, the Raji and Daudi cell surviving fractions of ^125I-UdR groups were （19.78 ± 1.39）% and （43.17±2.69）%; those of Na^125I groups were（ 79.10 ± 1.79）% and （80.36 ± 6.12）%. The surviving fractions of ^125I-UdR groups reduced with the ^125I -UdR concentration. Conclusions ^125I-UdR can be specially ingested by Raji and Daudi cells and incorporated into DNA, then the cells will be killed. The uptake of ^125I-UdR is dose and time dependent.