NSCs-HA-NT-3复合物移植修复兔面神经损伤

Repaire of rabbit facial nerve by the transplantation of NSCs-HA-NT-3 complex

目的观察NSCs-HA-Col支架-NT-3复合物对兔面神经完全横断损伤修复效果。方法新西兰大耳白兔41只,随机分为6组,E组为NSCs-HA支架-NT-3联合移植组。电生理于术前、术后1、4、8和12周记录肌电图的潜伏期、阈值、振幅。术后12周取桥接处神经,行B rdU及S100免疫组化染色,半薄切片及图像分析,透射电镜观察再生情况。结果E组术后12周可见较多B rdU阳性细胞,电生理检测与正常对照无显著性差异,桥接处纵断面呈现连续或不连续波浪状;横断面髓鞘结构与正常对照组的较为相似。结论NSCs-HA支架-NT-3复合物可以显著提高面神经损伤修复的效果。

Objective To explore the possibility of repairing injured facial nerve with tissue engineering technology and neural stem cells（NSCs）. The complex consisted of NSCs, scaffold and NT-3. NSCs were immature cells with the potential of self-renewal and multiple differentiation to neurons and glial cells. The scaffold with porous surface was made of hyaluronic acid and collagen （HA-Col gel） which degenerate in vivo after transplantation. NT-3 is the signal to promote neurons survival in vitro. Methods NSCs of S-D rat were co-cultured with scaffold and NT-3 in vitro. The two stumps of disconnected facial nerve of rabbit were re-connected with the complex. Electrophysiology and morphology tests were used to examine functional and morphological changes. Results Resultl NSCs adhered to the HA-Col gel and survived. Injured facial nerve fixed by NSCs-HA-Col gel-NT-3 complex showed significant improvement in function and anatomical structure. Conclusion Combinative implant of NSCs, HA-Col gel and NT-3 may promote the regeneration of injured facial nerve.